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目的采用尾静脉注射法初步建立白色假丝酵母菌感染所致严重脓毒血症动物模型,为进一步研究人类该疾病的发生、发展规律及预防、治疗提供实验依据。方法清洁级雄性Wister大鼠48只,随机分为6组,每组8只,对照组给予尾静脉注射生理盐水1ml/kg,其余5组给予尾静脉注射白色假丝酵母菌液1ml/kg(浓度为4×108集落形成单位/L)造成严重脓毒血症;分别于6、12、24、36、48h各时间点采血检测血常规及生化指标,并观察大鼠肝、心、肺、肾等组织病理学形态改变。结果各处理组血常规白细胞及生化指标、谷草转氨酶、尿素、肌酐与对照组均有显著差异,其中白细胞于6h开始逐渐降低,于24h时达最低值,之后有所上升;谷草转氨酶6h开始升高,12h升高明显,在24h时达高峰,之后逐渐下降;尿素和肌酐在6、12h变化不明显,在24h及之后有大幅度上升,48h达高峰,病理形态学检查显示动物多个器官明显受损。结论大鼠尾静脉注射白色假丝酵母菌液可致大鼠体内广泛炎症反应,并伴有多器官损伤,其改变符合严重脓毒血症的标准和特征,该研究成功建立白色假丝酵母菌脓毒症动物模型,为真菌脓毒血症研究奠定基础。
Objective To establish an animal model of severe sepsis induced by Candida albicans infection by tail vein injection and provide experimental evidence for further study of the occurrence, development, prevention and treatment of this disease. Methods Forty-eight clean-grade male Wister rats were randomly divided into 6 groups with 8 rats in each group. The control group was given normal saline (1ml / kg) through the tail vein and the other 5 groups were injected with Candida albicans solution 1ml / kg The concentration of 4 × 108 colony forming units / L) caused severe sepsis; Blood samples were collected at 6, 12, 24, 36, and 48 hours to determine blood and biochemical parameters, and liver, heart, lung, Kidney and other histopathological changes. Results The levels of leukocyte and biochemical indexes, aspartate aminotransferase, urea, creatinine and the control group were significantly different among the treatment groups. The leukocyte decreased gradually from 6h to the lowest at 24h and then increased. The aspartate aminotransferase began to rise at 6h High, increased significantly at 12h, peaked at 24h, and then decreased gradually. Urea and creatinine did not change significantly at 6 and 12h, increased significantly at 24h and later, and peaked at 48h. Pathological examination showed that multiple organs Obvious damage. Conclusion The rat caudal vein injection of Candida albicans liquid can cause a wide range of inflammation in rats with multiple organ damage, the changes consistent with the criteria and characteristics of severe sepsis, the successful establishment of Candida albicans Animal models of sepsis lay the foundation for the study of fungal sepsis.