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目的了解四川地区哨点医院轮状病毒腹泻流行状况和特点,为疫苗研制和疫情控制提供科学依据。方法四川监测点(成都市儿童医院)监测对象为5岁以下腹泻的门诊和住院患儿,收集2006-2010年患儿的粪便标本进行轮状病毒检测和分型,使用ELISA进行病毒检测,RT-PCR进行病毒分型。结果 972例腹泻患儿送检标本中检测到353株轮状病毒抗原阳性,阳性率为36.3%,6月龄-2岁幼儿是轮状病毒感染的高发年龄段,轮状病毒腹泻发病的高峰季节是10-12月份。结论轮状病毒是四川地区哨点医院婴幼儿腹泻的重要病原,毒株流行型别显示一定的规律性和多样性。
Objective To understand the prevalence and characteristics of rotavirus diarrhea in sentinel hospital in Sichuan Province and to provide a scientific basis for vaccine development and epidemic control. Methods The surveillance points in Sichuan (Chengdu Children’s Hospital) were out-patients and hospitalized children with diarrhea under 5 years of age. The stool specimens from 2006 to 2010 were collected for rotavirus detection and typing. The viruses were detected by ELISA. RT -PCR for virus typing. Results A total of 353 rotavirus positive samples were detected in 972 children with diarrhea. The positive rate was 36.3%. The children aged 6 months to 2 years old were the high incidence of rotavirus infection and the peak of rotavirus diarrhea The season is from October to December. Conclusions Rotavirus is an important pathogen of infantile diarrhea in sentinel hospital in Sichuan Province. The prevalence of strains shows some regularity and diversity.