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本文以高粱和向日葵为例介绍转导含目的性状种质总DNA创造农作物新资源的方法。1988年以高粱ICS-12B为受体,以高粱具矮秆及早熟性状的IS7518C和具抗蚜性的BTAM428总DNA为供体进行转导处理,F5得到含有不同目的性状的稳定系23个。1992年以大豆辽豆10号为外源DNA供体,转导处理高粱115等3个恢复系,从F2始选择籽粒蛋白质和赖氨酸含量均高于对照(未转导的受体)的个体组成下一代,2个恢复系F4的蛋白质和赖氨酸平均含量都比对照增加,115分别增长7.82%和3.61%,0-30分别增长20.27%和8.34%。1992年以菊芋为供体,将其总DNA导入向日葵保持系7718B中,F2和F3的接种鉴定结果显示Dj253和Dj273两个系对霜霉病(PL2)表现出完全免疫。
In this paper, sorghum and sunflower as an example to introduce the purpose of tracing the trait of the total DNA of the crop to create a new method of crop resources. In 1988, sorghum ICS-12B was used as receptor, IS7518C with dwarf and precocity of sorghum and total DNA of BTAM428 with aphid resistance were used as transducers. F5 was obtained with 23 stable lines with different traits. In 1992, Soybean Liaodou 10 was used as an exogenous DNA donor to transduce 3 restorer lines of sorghum 115. The content of grain protein and lysine were higher than those of the control (untransduced receptor) from F2 The average content of protein and lysine of the next generation and two restorer lines F4 increased by 7.82% and 3.61% respectively compared with the control, but increased by 20.27% and 8.34% respectively from 0-30, . In 1992, Jerusalem artichoke was used as a donor, and its total DNA was introduced into Helianthus annuus 7718B. Inoculation results of F2 and F3 showed that both strains of DJ253 and Dj273 showed complete immunity against downy mildew (PL2).