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表面等离子体共振(SPR)传感器已广泛应用于生物学、医学、化学、药物筛选以及环境监测、食品安全等领域。但SPR传感系统检测样品时易受到来自光源、光电检测器等器件自身噪声及环境光噪声的影响,其中环境光噪声的影响尤为明显,因此SPR传感器通常需要在遮光环境下进行操作,不利于现场的快速检测。为此,在自行研制的角度扫描型SPR传感器基础上,重点研究环境光噪声的特性,提出一种统计平均滤波和低通滤波结合的数据处理方法来抑制日光灯照环境下的噪声干扰。检测磷酸盐(PBS)缓冲液的实验结果表明,该方法可使SPR传感器在日光灯照环境下准确检测,相对遮光测量结果的误差为0.0027%。该方法可有效提高角度扫描型SPR传感器抗环境光干扰能力,具有良好的应用和推广价值。
Surface plasmon resonance (SPR) sensors have been widely used in biology, medicine, chemistry, drug screening and environmental monitoring, food safety and other fields. However, the SPR sensor system is susceptible to self-noise and ambient light noise from the light source, photodetector and other devices when testing the sample. The effect of ambient light noise is particularly significant, so the SPR sensor usually needs to operate in a shading environment, which is not good for The scene of rapid testing. Based on the self-developed angle-scanning SPR sensor, this paper focuses on the characteristics of ambient light noise, and proposes a data processing method combining statistical average filtering and low-pass filtering to suppress the noise interference under the fluorescent lighting environment. The experimental results of detecting phosphate buffered saline (PBS) showed that this method can accurately detect the SPR sensor under the fluorescent light environment, and the error of relative shading measurement is 0.0027%. The method can effectively improve the anti-ambient light interference ability of the angle-scanning SPR sensor, and has good application and promotion value.