【摘 要】
：
Glutathione (GSH) plays a critical role in maintaining cellular redox homeostasis in biological system.Mitochondrion is a pivotal organelle for cellular aerobic respiration and its disorder is associated with impaired redox balance, leading to cell death.
【机 构】
：
State Key Laboratory of Bioreactor Engineering,Shanghai Key Laboratory of Chemical Biology,School of
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Glutathione (GSH) plays a critical role in maintaining cellular redox homeostasis in biological system.Mitochondrion is a pivotal organelle for cellular aerobic respiration and its disorder is associated with impaired redox balance, leading to cell death. In this work, we designed and synthesized a non-invasive “off-on” mitochondrial-targeting fluorescent probe QZ for the detection of GSH in living cells. Based on the mechanism of native chemical ligation (NCL) and fluorescence resonance energy transfer (FRET), a rhodamine B derivative, QZ was prepared, by choosing aromatic thioester bond as the selective reaction site. QZ exhibited excellent detection capability for GSH over Cys and Hcy. Upon addition of GSH to QZ solution, a remarkably enhanced fluorescence was observed with a limit of detection of 2.98 μmol/L. Furthermore, QZ was found to possess the specific mitochondrial localization ability in cell imaging experiments.Moreover, with exogenous and endogenous stimulations, QZ could image GSH in living cells.
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