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目的:探讨灯盏花素预适应对大鼠肢体缺血再灌注肝损伤的作用。方法:将50只大鼠随机分为对照组、模型组、预适应组、灯盏花素组及灯盏花素预适应五组。分别测定各组大鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)的含量;肝组织匀浆丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性及肝组织病理学变化。结果:灯盏花素和预适应组均明显降低大鼠血清ALT、AST活性,灯盏花素预适应组较灯盏花素组更明显。预适应、灯盏花素和灯盏花素预适应组均明显降低肝匀浆MDA的含量,提高肝匀浆SOD的活性。肝组织病理学检查,灯盏花素预适应组明显减轻肝损伤。结论:与灯盏花素组比较,灯盏花素预适应对大鼠肢体缺血再灌注肝损伤具有明显的保护作用。
Objective: To investigate the effect of breviscapine preconditioning on liver injury following limbs ischemia-reperfusion in rats. Methods: Fifty rats were randomly divided into five groups: control group, model group, preconditioning group, breviscapine group and breviscapine preconditioning group. Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) ) Activity and liver histopathological changes. Results: Breviscapine and preconditioning group were significantly lower serum ALT, AST activity, Breviscapine pre-adaptive group was more obvious than breviscapine group. Preconditioning, breviscapine and breviscapine preconditioning group significantly reduced the content of MDA in liver homogenate and increased the activity of SOD in liver homogenate. Liver histopathology, breviscapine preconditioning group significantly reduced liver injury. Conclusion: Compared with breviscapine group, Breviscapine preconditioning has a significant protective effect on liver ischemia-reperfusion injury in rats.