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用添加绿色荧光蛋白(GFP)饲料投喂牙鲆,研究了不同消化时间内食糜在牙鲆消化道中的分布情况,采用福林-酚法测定了不同时间胃、前肠、中肠和后肠各组织黏液蛋白酶活性。结果表明,牙鲆在摄食后消化道蛋白酶活性随着食糜在消化道内的分布而变化,食物的刺激引起消化腺体分泌蛋白酶。牙鲆摄食后1h食糜达到中肠,胃和中肠蛋白酶活性与空腹相比迅速增高;食糜在5h时遍布整个消化道,此时胃蛋白酶活性几乎检测不出,而前肠蛋白酶活性迅速增高,5h达到最高,然后开始降低;8h时中肠蛋白酶活性一直很高,之后降低;而后肠直到12h其蛋白酶活性仍维持在较高水平,说明牙鲆这种消化道较短的鱼类其肠道比胃在蛋白质消化过程中发挥更重要的作用。
The diet of green florescent protein (GFP) was fed on the Japanese flounder to study the distribution of the digesta in digestive tract of flounder during different digestion periods. The gastric, foregut, midgut and postmortem Intestinal mucosal protease activity. The results showed that digestive tract protease activity of Japanese flounder changed with the distribution of chyme in the digestive tract. The stimulation of food caused the digestive gland to secrete protease. Paralichthys olivaceus reached the midgut at 1 h after feeding, and the activity of protease in the stomach and midgut rapidly increased compared with fasting; chyme spread throughout the digestive tract at 5 h, at which point pepsin activity was almost undetectable and the activity of preproteinase was rapidly Increased at 5h, then began to decrease. At 8h, the activity of midgut protease was high and then decreased. The activity of protease remained high after 12h in the hindgut, indicating that the flounder is a kind of fish with shorter digestive tract The gut plays a more important role than the stomach in protein digestion.