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目的：观察参麦注射液对大鼠缺血心肌细胞内钙超载的影响及其心肌保护作用。方法：用异丙肾上腺素制备大鼠心肌缺血模型，Ｆｕｒａ－２ 法测定大鼠红细胞胞浆游离钙浓度（ＥｒｙＣａｉ）；生化法测定红细胞膜钙泵（Ｃａ－ｐｕｍ ｐ）、钠泵（Ｎａ－ｐｕｍ ｐ）活性，同步观察大鼠心肌组织病理变化。结果：参麦注射液治疗后ＥｒｙＣａｉ较缺血组显著降低（１．６８±０．１０ Ｆ３３５／Ｆ３８５ ｖｓ１．５６±０．１５ Ｆ３３５／Ｆ３８５ Ｐ＜ ０．０５），但仍高于对照组（１．５６±０．１５ Ｆ３３５／Ｆ３８５ ｖｓ１．３６±０．１０Ｆ３３５／Ｆ３８５ Ｐ＜ ０．００１）；钙泵（１０５．１±２９．４ μｍ ｏｌＰｉ·ｇＨｂ－ １ ·２ｈ－ １ｖｓ １２６．８±３０．７ μｍ ｏｌＰｉ·ｇＨｂ－ １ ·２ｈ－ １ Ｐ＞ ０．０５）及钠泵（３５．１±１８．２ μｍ ｏｌＰｉ·ｇＨｂ－ １·２ｈ－ １ ｖｓ ４３．３±１０．２ μｍ ｏｌＰｉ·ｇＨｂ－ １·２ｈ－ １ Ｐ＞ ０．０５）活性较缺血组有所增高，但无统计学意义，且钙泵（１２６．８±３０．７ μｍ ｏｌＰｉ·ｇＨｂ－ １ ·２ｈ－ １ ｖｓ １６８．６±３９．６ μｍ ｏｌＰｉ·ｇＨｂ－ １ ·２ｈ－ １ Ｐ＜ ０．０１）及钠泵（４３
OBJECTIVE: To observe the effect of Shenmai injection on intracellular calcium overload in ischemic myocardium and its myocardial protection. METHODS: The rat model of myocardial ischemia was established with isoproterenol, the cytoplasmic free calcium concentration (EryCai) was determined by Fura-2 method, and the red blood cell membrane calcium pump (Ca-pum p) and sodium pump (Na) were measured by biochemical method. -pum p) activity, synchronous observation of myocardial tissue pathological changes. RESULTS: EryCai was significantly lower in Shenmai injection than in the ischemic group (1.68±0.10 F335/F385 vs1.56±0.15 F335/F385 P<0.05), but it was still higher than the control group ( 1.56±0.15 F335/F385 vs1.36±0.10F335/F385 P<0.001); Calcium Pump (105.1±29.4 μm olPi·gHb-1 ·2h- 1 vs 126.8±) 30.7 μm olPi·gHb-1 · 2h- 1 P> 0.05) and sodium pump (35.1 ± 18.2 μm olPi·gHb-1 · 2h-1 vs 43.3 ± 10.2 μm olPi ·gHb-1 · 2h-1 · P> 0.05) activity was increased compared with the ischemic group, but was not statistically significant, and the calcium pump (126.8 ± 30.7 μm olPi · gHb-1 · 2h-1 Vs 168.6±39.6 μm olPi·g Hb-1 · 2h- 1 P< 0.01) and sodium pump (43