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目的 探讨壳聚糖及衍生物在大鼠脑内的生物相容性,筛选出适宜的药物载体。方法 将壳聚糖及衍生物混悬液注射入脑内,分别在3、7、14、3 0d检测血清神经元特异性烯醇化酶(NSE)和S 10 0蛋白水平,同时作病理学检查,并用生理盐水作对照。结果 第3、7、14、3 0天壳聚糖组NSE和S 10 0蛋白水平与生理盐水组比较差异均无统计学意义(P >0 .0 5 ) ,高黏度壳聚糖组几乎均高于生理盐水组(P <0 .0 5 ) ,且在7、14d高于壳聚糖组(P <0 .0 5 )。羧甲基壳聚糖组NSE水平7d高于壳聚糖组(P <0 .0 5 ) ,14d高于生理盐水组(P <0 .0 5 )。光镜下壳聚糖组反应与生理盐水组类似,高黏度壳聚糖组与羧甲基壳聚糖组胶质细胞增生明显,早中期有脑水肿,且降解迟缓。结论 壳聚糖对神经系统损害轻微,具有良好的生物相容性,羧甲基壳聚糖次之
Objective To investigate the biocompatibility of chitosan and its derivatives in rat brain and select suitable drug carriers. Methods The suspension of chitosan and its derivatives were injected into the brain. Serum levels of neuron specific enolase (NSE) and S 10 0 were detected at 3, 7, 14 and 30 days respectively. The pathological examination , And saline control. Results There was no significant difference in the NSE and S 10 protein levels between the chitosan group and the saline group on the 3rd, 7th, 14th and 30th days (P> 0.05) Which was higher than that of saline group (P <0.05), and higher than that of chitosan group at 7 and 14 d (P <0.05). The level of NSE in carboxymethyl chitosan group was higher than that in chitosan group (P <0.05), and higher than that in saline group at 14th day (P <0.05). Under the light microscope, the reaction of chitosan group was similar to that of saline group. Glial cell hyperplasia was observed in high viscosity chitosan group and carboxymethyl chitosan group, with cerebral edema in early and mid-term, with slow degradation. Conclusion Chitosan has slight damage to the nervous system, has good biocompatibility, followed by carboxymethyl chitosan