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目的:分析6种角膜接触镜多功能护理液和加入甲硝唑滴眼液的护理液对自生生活性棘阿米巴原虫的杀伤效果。方法:将6种多功能护理液分别加入96孔板中,每种护理液占用48孔,其中24孔滴入阿米巴悬液,另外24孔先滴入甲硝唑滴眼液后再滴入阿米巴悬液,室温静置8h后在倒置显微镜下观察残存阿米巴的形态变化和数量。将残存的棘阿米巴原虫分别在PYG培养液中培养5d,观察其形态、活性与增殖能力的变化。结果:单纯护理液组1-6号阿米巴检出率分别为0%、80.3%、29.1%、41.7%、62.5%、79.2%,加入甲硝唑滴眼液后护理液1-6号阿米巴检出率分别为0%、0%、4.2%、8.3%、16.7%、16.7%,3-6号护理液加与不加甲硝唑滴眼液杀伤阿米巴的效力差异有统计学意义(3-6组的χ2值分别为3.75、7.11、10.54和18.78,P<0.05)。残存的棘阿米巴原虫经培养后活力与增殖力减弱。结论:部分多功能护理液对棘阿米巴原虫的杀伤效果不佳,添加甲硝唑滴眼液后杀伤效果明显提高。
Objective: To analyze the killing effects of six kinds of multi-functional contact lens solution and metronidazole eye drops on spontaneous Acanthamoeba sp. Methods: Six kinds of multi-functional care solution were added to 96-well plates, each care solution occupies 48 holes, of which 24 were dripped amoeba suspension, and the other 24 first metronidazole eye drops drip drip Into the amoeba suspension, after standing at room temperature for 8h under inverted microscope to observe the morphological changes and the amount of residual amoeba. The remaining Acanthamoeba were cultured in PYG medium for 5 days to observe the morphological changes, activity and proliferation ability. Results: The detection rates of amoeba in group 1-6 were 0%, 80.3%, 29.1%, 41.7%, 62.5% and 79.2%, respectively. After adding metronidazole eye drops, Amoeba detection rates were 0%, 0%, 4.2%, 8.3%, 16.7%, 16.7%, 3-6 nursing solution plus metronidazole eye drops efficacy of killing amoeba differences are Statistical significance (χ2 values in Groups 3-6 were 3.75, 7.11, 10.54 and 18.78 respectively, P <0.05). Residual Acanthamoeba cultured viability and proliferation decreased. Conclusion: Some of the multi-functional care solutions have a poor killing effect on Acanthamoeba sp., And the killing effect is obviously improved after adding metronidazole eye drops.