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长期液体培养如前所述,迄今多数有关造血干细胞的研究,采用半固体培养进行细胞对外源性因子反应的观察,因属短期培养,缺乏对多向性造血千细胞的自我更新,细胞之问的相互作用,或造血干细胞与微环境的关系;以及造血干细胞增生与分化的动力学等的观察。Mc Culloch等1971年用肾脏作为滋养细胞,Sumner 等1972年用鼠胚或妊娠鼠子宫提取物,Testa 等1973年用胚胎条件培养液进行液体培养,发现大多数CFU—s,与GM—CFUc 迅速消失。Dexter 1974年用鼠胸腺细胞与骨髓细胞同时进行液体培养,则CFU—S 与GM—CFUc 数周后始消失。Dexter 1976、1977年始成功地建
Long-term liquid culture As mentioned above, so far most of the research on hematopoietic stem cells, the use of semi-solid culture cell exogenous factor reaction observation, because of short-term culture, lack of multi-directional hematopoietic stem cell self-renewal, cell questions Or the relationship between hematopoietic stem cells and microenvironment, as well as the observation of kinetics of hematopoietic stem cell proliferation and differentiation. Mc Culloch and other kidneys as trophoblastic cells in 1971, Sumner and other 1972 rat embryo or pregnant rat uterine extract, Testa and other embryos in 1973 conditioned medium for liquid culture and found that most of the CFU-s, and GM-CFUc rapid disappear. Dexter 1974 with murine thymocytes and bone marrow cells were cultured in liquid, then CFU-S and GM-CFUc disappear after a few weeks. Dexter 1976, successfully built in 1977