论文部分内容阅读
目的探讨结合偶联半乳糖（Gal）的大鼠抗小鼠CD3单克隆抗体（Mouse-anti-rst—CD3monocloalantibodyAntiCD3-McAb）肿瘤浸润性淋巴细胞的（TIL）趋肝性，方法本实验把大鼠抗小鼠CD3单克隆抗体和半乳糖（Gal）偶联在一起，与3H-TdR标记TIL结合后，从尾静脉注入小鼠体内，分别在注射后不同时间抠眼取血0．5ml，然后处死，切除肝、脾、肺组织，分别称重后进行放射性强度测定。结果结果显示：注射Gal-anti-CD3-McAb-TIL小鼠肝脏组织比注射单纯TIL的小鼠肝脏组织具有较高的放射性强度（P<0.001），且该放射性持续较长一段时间，而脾、肺、血组织内放射性强度无明显差异（P>0．5P>0.5P>0．2）。结论该结果提示Gal-anti-CD3-McAb-TIL较单纯TIL具有更良好的趋肝性，本实验为肝脏恶性肿瘤生物导向治疗和靶向性基因治疗提供了新的方法。
OBJECTIVE To investigate the effect of galactose-conjugated galactose (Gal) rat anti-mouse CD3 monoclonal antibody (TIL) on liver infiltration of tumor infiltrating lymphocytes (TIL). The anti-mouse CD3 monoclonal antibody was conjugated to galactose (Gal) and bound to the 3H-TdR-labeled TIL and injected into the mice from the tail vein. Blood was taken at 0.5 ml each time after the injection. They were sacrificed and liver, spleen, and lung tissues were excised and weighed and measured for radioactivity. The results showed that the liver tissue of mice injected with Gal-anti-CD3-McAb-TIL had higher radioactivity intensity than that of mice injected with TIL alone (P<0.001), and the radioactivity continued for a long period of time. There was no significant difference in the intensity of radioactivity between lung, lung and blood (P>0.5P>0.5P>0.2). Conclusion This result suggests that Gal-anti-CD3-McAb-TIL has better hepatotropic properties than pure TIL. This experiment provides a new method for the biological targeted therapy and targeted gene therapy of liver malignancies.