论文部分内容阅读
目的 研究p2 7及p2 1在血管平滑肌细胞增殖中的作用。方法 分离SD大鼠主动脉中层平滑肌,培养平滑肌细胞。实验前无血清培养基培养4 8h ,使细胞进入静止期。以无血清组为对照组,培养基中分别加入血小板源性生长因子-BB(PDGF -BB) 2 0 μg/L和AngⅡ10 -6M ,分别在刺激6 ,12 ,2 4h收集细胞。采用Western蛋白印记法,检测平滑肌细胞内p2 7和p2 1蛋白表达量。结果 与AngⅡ组比较,PDGF -BB组p2 7蛋白表达量明显下降,刺激2 4hp2 7蛋白表达量下降幅度最大,较对照组降低5 9.32 % ;AngⅡ组p2 7光密度值与对照组相近,比较无显著性差异。PDGF -BB组p2 1蛋白表达量则升高,刺激2 4h时PDGF -BB组光密度值较对照组增加了6 6 .95 % ,较AngⅡ组增加了10 1.14 % ,PDGF -BB组与对照组和AngⅡ组比较均有显著性差异(P <0 .0 1) ;AngⅡ组较对照组减少17% ,2组比较无显著性差异。结论 p2 7蛋白表达量在血管平滑肌细胞增殖中降低,而p2 1蛋白表达量升高,两者在血管平滑肌细胞增殖中的作用不同。
Objective To study the role of p27 and p21 in the proliferation of vascular smooth muscle cells. Methods The aortic smooth muscle of SD rats was isolated and cultured. Before the experiment, serum-free medium was incubated for 48 h, allowing the cells to enter the quiescent phase. The serum-free group served as the control group. Platelet-derived growth factor-BB (PDGF-BB) 20 μg / L and Ang Ⅱ 10 -6M were respectively added to the culture medium. Cells were harvested at 6, 12 and 24 hours respectively. Western blotting was used to detect the expression of p2 7 and p21 in smooth muscle cells. Results Compared with Ang Ⅱ group, the expression of p27 protein in PDGF-BB group decreased significantly, and the expression of 24 hp27 protein in stimulated group decreased the most, which was 5 9.32% lower than that in control group. The optical density of p27 in AngⅡ group was similar to that in control group No significant difference. PDGF-BB group p2 1 protein expression increased, stimulated PDGF-BB group 24 hours when compared with the control group optical density value increased by 6 6.95%, compared with Ang Ⅱ group increased by 10 1.14% PDGF -BB group and control There was a significant difference between AngⅡgroup and AngⅡgroup (P <0.01). There was no significant difference between AngⅡgroup and control group (17%). Conclusion The expression of p27 protein is decreased in the proliferation of vascular smooth muscle cells, while the expression of p21 protein is increased. The role of p27 in vascular smooth muscle cell proliferation is different.