论文部分内容阅读
【目的】探讨促红细胞生成素(erythropoietin,EPO)对常压窒息新生大鼠脑组织内Omi/HtrA2表达水平及细胞凋亡的影响。【方法】7日龄新生SD大鼠随机分为对照组、窒息组和EPO干预组(每组n=25)。对照组模拟窒息过程但不缺氧,窒息组和EPO干预组制备新生大鼠常压窒息模型,EPO干预组窒息模型制备后即刻腹腔注射重组人促红细胞生成素5 000U/kg,对照组和窒息组均注射同体积生理盐水。各组于造模后不同时间点(6、12、24、48、72h)取脑组织行石蜡切片,HE染色观察脑组织病理改变,TUNEL法检测神经细胞凋亡,免疫组化法检测Omi/HtrA2表达。【结果】窒息组脑组织Omi/HtrA2的表达和凋亡细胞数在各时间点均高于对照组(P均<0.05),而EPO干预组同窒息组比较,各时间点凋亡细胞数和Omi/HtrA2表达均显著下降,其差异均有统计学意义(P均<0.05),但均未恢复至对照组水平(P<0.05)。【结论】EPO可能通过抑制脑组织Omi/HtrA2的表达而减少神经细胞的凋亡,从而对窒息脑损伤起到保护作用。
【Objective】 To investigate the effects of erythropoietin (EPO) on the expression of Omi / HtrA2 and the apoptosis in neonatal rats with asphyxia. 【Methods】 Newborn SD rats of 7 days old were randomly divided into control group, asphyxia group and EPO intervention group (n = 25 each group). In the control group, the asphyxia process was simulated but not in hypoxia. The asphyxia group and the EPO intervention group were given asphyxia model of neonatal rats. The asphyxial model of EPO intervention group was injected intraperitoneally with 5000U / kg of recombinant human erythropoietin. The control group and asphyxia Group were injected with the same volume of saline. The rats in each group were sacrificed at different time points (6, 12, 24, 48 and 72h). The brain tissues were paraffin sectioned and stained with hematoxylin and eosin (HE) for histopathological changes. Neuronal apoptosis was detected by TUNEL method. Omi / HtrA2 expression. 【Results】 The expression of Omi / HtrA2 and the number of apoptotic cells in the asphyxia group were higher than those in the control group at each time point (P <0.05). However, compared with the asphyxia group, the number of apoptotic cells and Omi / HtrA2 expression were significantly decreased, the difference was statistically significant (P all <0.05), but did not recover to the level of the control group (P <0.05). 【Conclusion】 EPO may reduce the apoptosis of nerve cells by inhibiting the expression of Omi / HtrA2 in brain tissue, which may play a protective role in asphyxial brain injury.