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目的:探讨市售中药壁虎的三种剂型散剂、水煎液和酒剂对S180荷瘤小鼠的作用。方法:建立移植瘤小鼠S180肉瘤模型和S180腹水瘤模型。将90只ICR小鼠和90只昆明种(KM)小鼠随机分为模型组,酒对照组,环磷酰胺(CTX)组,壁虎水煎液、散剂和酒剂高、低剂量组,分别以生理盐水、56度白酒及不同剂型的壁虎灌服荷瘤小鼠10d(CTX组20mg/kg,连续5次),测定抑瘤率、胸腺指数、脾指数和瘤组织血管内皮生长因子(VEGF)含量,对腹水瘤模型KM小鼠进行生存期分析。结果:与模型组比较,本实验所使用的市售中药壁虎各剂型对S180移植瘤模型的瘤重、瘤组织VEGF含量及生存期无明显影响,9组小鼠的胸腺及脾指数没有明显差别。结论:目前市售中药材壁虎的体内抑瘤活性尚不确定,影响壁虎抗肿瘤活性的相关因素有待进一步的研究。
OBJECTIVE: To investigate the effect of three kinds of powder, water decoction and alcohol on commercial S180 tumor-bearing mice. Methods: To establish S180 sarcoma model and S180 ascites tumor model in xenografted mice. 90 ICR mice and 90 Kunming mice were randomly divided into model group, alcohol control group, cyclophosphamide (CTX) group, gecko decoction, powder and alcohol high and low dose groups The tumor-bearing rate, thymus index, spleen index and the expression of vascular endothelial growth factor (VEGF) in tumor tissue were determined by injecting the tumor-bearing mice with normal saline, geoduck with 56 degree liquor and different gecko species for 10 days (CTX group 20mg / ) Content, ascites tumor model of KM mice survival analysis. Results: Compared with the model group, the gecko of commercial Chinese gecko used in this experiment had no significant effect on the tumor weight, tumor VEGF content and survival of the S180 xenograft model. There was no significant difference in the thymus and spleen index between the 9 groups . Conclusion: At present, the antitumor activity of Gekko gecko on the market is uncertain. The related factors that affect gecko antineoplastic activity need to be further studied.