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反义核酸技术具有实际应用的价值,它可选择性地阻断特定基因的表达,期望能用于病毒性疾病、肿瘤等相关疾病的基因治疗,也进行了实验性探索。在用反义技术抑制肿瘤细胞中激活的癌基因时,通常的设计并未考虑其特殊的突变序列而选用与野生型序列互补的反义寡核苷酸,这种设计就存在缺陷。本研究观察了反义核苷酸对抑制大肠癌细胞激活的ras基因表达及其生长的序列特异性。另一方面,采用完全互补的反义核酸阻断人类错配识别蛋白基因hMSH2的表达,分析细胞表型的变化,探索该基因缺陷的直接后果,以明确人类因hMSH2遗传性
Antisense nucleic acid technology is of practical value. It can selectively block the expression of specific genes and is expected to be used in gene therapy of diseases related to viral diseases and tumors. Some experimental exploration has also been carried out. When antisense technology is used to inhibit oncogenes that are activated in tumor cells, the design is usually flawed without considering its particular mutation sequence and using antisense oligonucleotides complementary to the wild-type sequence. In this study, we observed the sequence specificity of antisense oligonucleotides for inhibiting the expression of ras gene and its growth in colorectal cancer cells. On the other hand, completely complementary antisense nucleic acid is used to block the expression of human mismatch recognition protein gene hMSH2, analyze the change of cell phenotype, and explore the direct consequence of the defect of the gene so as to make clear that human beings inherit hMSH2 genetic