论文部分内容阅读
目的 :观察成年小鼠和老年小鼠腹腔巨噬细胞HSP7O .iNOS及iNOSmRNA表达的情况 ,探讨成年和老年机体免疫功能差异的机制 ,为老年医疗保健提供理论依据。方法 :取成年小鼠和老年小鼠各 10只 ,取小鼠腹腔巨噬细胞制作标本 ,采用免疫组化、组织化学、原位杂交及完整细胞斑点印迹技术检测HSP70、iNOS及iNOSm RNA的表达 ,并对所有斑点印迹信号进行扫描 ,其扫描值采用t检验进行统计学处理。结果 :成年小鼠HS7O .i NOSmRNA的表达为 7.13± 2 .12、5.57± 1.36及 12 .39± 2 .6 8,老年小鼠HSP70 .iNOS及iNOSmRNA的表达为 4 .34± 1.57、3.0 3± 1.2 8及 5.89± 1.90 ,成年小鼠和老年小鼠相比有显著差异 ,P <0 .0 1。结论 :提示老年机体免疫功能低下的机制之一可能与机体表达HSP70 .iNOS及iNOSmRNA的下降有关。
OBJECTIVE: To observe the expression of HSP70, iNOS and iNOSmRNA in peritoneal macrophages in adult and aged mice, and to explore the mechanism of immune function differences between adult and aged mice and to provide a theoretical basis for geriatric health care. Methods: Ten adult and aged mice were selected and their peritoneal macrophages were used to make specimens. The expression of HSP70, iNOS and iNOSm RNA were detected by immunohistochemistry, histochemistry, in situ hybridization and whole cell dot blotting , And all dot blot signal was scanned, the scan value using t test for statistical analysis. Results: The expression of HS7O.i NOSmRNA in adult mice was 7.13 ± 2.12,5.57 ± 1.36 and 12.39 ± 2.06, respectively. The expression of HSP70, iNOS and iNOS mRNA in aged mice was 4.34 ± 1.57,3.0 3 ± 1.2 8 and 5.89 ± 1.90, there was a significant difference between adult and old mice (P <0.01). Conclusion: One of the mechanisms that suggest that the immune function of the elderly is low may be related to the decrease of the expression of HSP70, iNOS and iNOS mRNA.