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建立ＭＴＴ（３－（４，５－ｄｉｍｅｔｈｙｌｔｈｉａｚｏｌ－２－ｙｌ）－２，５－ｄｉｐｈｅｎｙｌ－ｔｅｔｒａｚｏｌｉｕｍｂｒｏｍｉｄｅ）比色法进行血小板免疫活性的测定。最适血小板浓度为１０７／孔，最适ＭＴＴ浓度为４ｍｇ／ｍｌ（８０μｇ／孔）。ＩｇＥ依赖组与阿斯匹林依赖组血小板活性测定ＭＴＴ比色法与以往的血小板介导的杀寄生虫幼虫的细胞毒素测定方法呈显著相关（ＩｇＥ依赖组两者的相关系数ｒ＝０９５１，阿斯匹林依赖组两者的相关系数ｒ＝０８０２）。实验表明，ＭＴＴ比色法因其方法简便、准确、试剂易得，可作为临床检测血小板活性的一种实用方法
The establishment of MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazoliumbromide) assay for platelet immunocompetence. The optimal platelet concentration was 107 / well, and the optimal MTT concentration was 4mg / ml (80μg / well). MTT colorimetric assay was significantly associated with platelet-mediated killing of parasitic larvae in the IgE-dependent and aspirin-dependent groups (IgE-dependent group, r = 0.951 , Aspirin dependence group correlation coefficient r = 0802). Experiments show that, MTT colorimetric method because of its simple, accurate, easy to get reagents, as a clinical test platelet activity of a practical method