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将一系列核心启动子区的缺失突变引入乙肝病毒（ Ｈ Ｂ Ｖ） 线性转录单元， 从病毒的抗原、 Ｒ Ｎ Ａ 以及子代 Ｄ Ｎ Ａ 等各个水平， 分析了各缺失突变对前基因组 Ｒ Ｎ Ａ 和前核心 Ｒ Ｎ Ａ 转录的影响， 对核心启动子各片段的功能进行了深入的研究。 Ｃ 片段缺失后检测不到ｅ 抗原和前核心 Ｒ Ｎ Ａ， 却仍有核心抗原和前基因组 Ｒ Ｎ Ａ 的合成以及病毒子代 Ｄ Ｎ Ａ 的复制； 而 Ｂ３ 片段缺失后ｅ 抗原和核心抗原均有显著下降， 但仍能检测到两种ｍ Ｒ Ｎ Ａ 的合成和病毒子代 Ｄ Ｎ Ａ。结果表明， 核心启动子区域的 Ｃ 片段和 Ｄ 片段均具有基本启动子功能， 它们介导前核心 Ｒ Ｎ Ａ 和前基因组 Ｒ Ｎ Ａ 的基础转录。 Ｄ 片段对前基因组转录起始的重要作用尚未见报道。上游与增强子 Ｉ Ｉ重叠的 Ｂ３ 片段对这两种 Ｒ Ｎ Ａ 的有效转录起重要的调节作用
A series of deletion mutations in the core promoter region were introduced into the linear transcription unit of hepatitis B virus (HBV). From the antigen of the virus, R N A and the descended D N A, A and pre-core R N A transcripts, the functions of the core promoter fragments were studied in depth. However, the e antigen and pre-core R N A were not detected after the deletion of C fragment, but the synthesis of core antigen and pre-genomic R N A and the replication of virus progeny D N A still remained. However, both e antigen and core antigen There was a significant decrease, but both the synthetic and viral progeny of the m R N A, D N A, were still detectable. The results showed that both the C and D fragments in the core promoter region have basic promoter functions that mediate the basal transcription of R N A in the pre-core and R N A in the pre-genome. The important role of D fragment on the initiation of pregenome transcription has not been reported yet. The B3 fragment upstream overlapping with enhancer I I plays an important regulatory role in the efficient transcription of both R N A