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目的研究乌饭树Vaccinium bracteatum叶化学成分及其抗补体活性。方法通过溶血实验,以抗补体活性作为导向分离手段,对乌饭树叶各部位进行抗补体活性测试,运用多种色谱方法进行分离纯化,采用现代谱学技术鉴定化合物结构,确定抗补体活性成分。结果从乌饭树叶70%乙醇提取物中分离得到28个化合物,分别鉴定为三十五烷(1)、三十一烷(2)、赤杨酮(3)、木栓酮(4)、表木栓醇(5)、羽扇豆醇(6)、齐墩果酸(7)、熊果酸(8)、东莨菪素(9)、反式对羟基桂皮酸(10)、柯伊利素(11)、芹菜素(12)、山柰酚(13)、山楂酸(14)、科罗索酸(15)、蔷薇酸(16)、2α,3α-二羟基熊果酸(17)、19α-羟基熊果酸(18)、咖啡酸(19)、异荭草苷(20)、荭草苷(21)、牡荆苷(22)、异牡荆苷(23)、槲皮素-3-O-α-L-鼠李糖苷(24)、异槲皮苷(25)、柯伊利素-7-O-β-D-葡萄糖苷(26)、槲皮素(27)、木犀草素(28)。对其中的25个化合物进行抗补体活性实验,化合物5、7、8、11~13、15、18~20、23~25、27、28对经典途径的补体激活具有不同程度的抑制作用。结论化合物1、3、6、14~18、23、25为首次从该植物中分离得到,熊果酸的抗补体活性最强,50%抑制溶血浓度(CH50)为0.014 mg/mL。
Objective To study the chemical constituents of leaves of Vaccinium bracteatum and their anti-complement activity. Methods The anti-complement activity was tested by anti-complement activity as a means of separation of hemolysis by hemolysis test. The anti-complement activity was tested by various chromatographic methods. The structures of compounds were identified by modern spectroscopy and the anti-complement active ingredients were determined. Results Twenty-eight compounds were isolated from 70% ethanol extract of the leaves of Radix angustifolium and were identified as pentacosane (1), triacontane (2), erythroidone (3), xylitol (4) Epiclodextrin (5), lupeol (6), oleanolic acid (7), ursolic acid (8), scopolamine (9), transhydroxycinnamic acid (10) (11), apigenin (12), kaempferol (13), maslinic acid (14), corosolic acid (15), rose acid (16), 2α, 3α-dihydroxy ursolic acid (18), caffeic acid (19), isoorientin (20), orientin (21), vitexin (22), isovitexin (23), quercetin- 3-O-α-L-rhamnoside (24), isoquercitrin (25), krelin-7-O-β-D-glucoside (26), quercetin (27) Prime (28). The anti-complement activity of 25 of these compounds was tested. Compounds 5, 7, 8, 11-13, 15, 18-20, 23-25, 27 and 28 inhibited the complement activation of classical pathway to some extent. Conclusion Compounds 1, 3, 6, 14-18, 23 and 25 were isolated from this plant for the first time. Ursolic acid had the highest anti-complement activity and the 50% inhibitory concentration (CH50) was 0.014 mg / mL.