论文部分内容阅读
本研究以子宫颈角化上皮为靶细胞，用试管培养技术及非同位素细胞毒性试验，探讨了干扰素（ＩＦＮ－γ）对淋巴细胞介导的对宫颈角化上皮细胞毒作用的影响及细胞间吸附分子１（ＩＣＡＭ－１）与白细胞功能相关抗原１（ＬＦＡ－１）在细胞毒作用中的意义。结果显示ＣａＳｋｉ、ＳｉＨａ、ＨｅＬａ、Ｗ１２及ＮＣｘ均为ＮＫ抵抗、ＬＡＫ敏感细胞。ＩＦＮ－γ预处理靶细胞明显增加ＬＡＫ细胞的杀伤活性，但对ＮＫ活性影响不大；ＩＦＮ－γ预处理效应细胞可增强ＬＡＫ细胞对上述靶细胞的溶解作用，但并不改变ＮＫ对靶细胞的选择性；抗ＩＣＡＭ－１与ＬＦＡ－１单克隆抗体能有效地降低ＬＡＫ细胞对靶细胞的杀伤作用，而抗ＨＬＡＡＢＣ及ＨＬＡＤＲ则无此作用。提示ＩＦＮ－γ仅对ＬＡＫ细胞介导的对宫颈角化上皮的细胞毒作用有明显增强作用，而对ＮＫ细胞无明显影响；ＩＣＡＭ－１－ＬＦＡ－１通路为ＬＡＫ细胞结合并溶解靶细胞的主要通路，且这种细胞毒作用是非ＭＨＣ限制的。
In this study, the keratinized epithelial cells of the cervix were used as target cells. The effects of interferon (IFN-γ) on lymphocyte-mediated cytotoxicity on cervical keratinized epithelial cells were investigated by in vitro culture and non-isotope cytotoxicity assay. The Significance of Intercellular Adhesion Molecule 1 (ICAM-1) and Leukocyte Function-Related Antigen 1 (LFA-1) in Cytotoxicity. The results show that CaSki, SiHa, HeLa, W12 and NCx are NK resistance, LAK sensitive cells. IFN-γ pretreatment target cells significantly increased LAK cell killing activity, but had little effect on NK activity; IFN-γ pretreatment effector cells could enhance LAK cell lysis of the target cells, but does not change the NK target cells The anti-ICAM-1 and LFA-1 monoclonal antibodies can effectively reduce the killing effect of LAK cells on target cells, while anti-HLA-ABC and HLADR do not. It is suggested that IFN-γcan only obviously enhance LAK cell-mediated cytotoxicity on cervical keratinized epithelial cells, but has no obvious effect on NK cells. ICAM-1-LFA-1 pathway binds LAK cells and lyses target cells Major pathway, and this cytotoxicity is non-MHC-restricted.