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本工作应用核团灌流液的放射免疫测定（ＲＩＡ），高压液相（ＨＰＬＣ）以及核团内注射拮抗剂，观察了蓝斑（ＬＣ）在刺激下丘脑视上核（ＳＯＮ）引起的加强电针（ＥＡ）镇痛效应中的作用。结果表明：化学刺激ＳＯＮ后电针期间和停针后３０、６０ｍｉｎ，蓝斑灌流液内ＯＴ的含量明显升高，刺激ＳＯＮ后电针期间灌流液内精氨酸加压素（ＡＶＰ）含量明显升高，停针后ＡＶＰ的含量虽高于对照组及注射前的水平，但无统计学意义。ＬＣ灌流液内去甲肾上腺素（ＮＥ）的含量在刺激ＳＯＮ后ＥＡ和停针后均明显低于人工脑脊液（ＡＣＳＦ）对照组和注射前的ＮＥ含量，但与对照组相比无差异，ＬＣ灌洗液内５－羟色胺（５—ＨＴ）的含量仅在刺激ＳＯＮ后ＥＡ期间，高于注射前各组及ＡＣＳＦ组和ＡＣＳＦ＋ＥＡ对照组，停针后则恢复到对照水平：ＬＣ内注射加压素Ｖ２受体拮抗剂对刺激ＳＯＮ引起的加强电针镇痛效应有轻度抑制作用，Ｖ１拮抗剂无明显影响，催产素拮抗剂可完全阻断这种作用，根据以上结果进行了讨论。
In this work, radioimmunoassay (RIA), high pressure liquid chromatography (HPLC) and intranuclear injection of antagonist were used in the nucleus perfusate to observe the effect of locus coeruleus (LC) stimulation on supraoptic nucleus (SON) Role of needle (EA) in analgesic effect. The results showed that the contents of OT in the locus coeruleus fluid after electroacupuncture at 30 and 60 min after electroacupuncture stimulation were significantly increased, and the content of arginine vasopressin (AVP) in the perfusion fluid after electroacupuncture stimulation was significantly increased However, the content of AVP in control group and pre-injection group was higher than that in control group and non-injection group, but it was not statistically significant. The content of norepinephrine (NE) in LC perfusion fluid was significantly lower than that of artificial cerebrospinal fluid (ACSF) and NE before injection after stimulation of EA and after needle-stop, but there was no difference compared with control group The content of 5-hydroxytryptamine (5-HT) in the lavage fluid was higher than that of the pre-injection group and the ACSF group and the ACSF + EA control group only after stimulation of EA, and returned to the control level after the needle was de-activated Angiotensin II receptor antagonists have a mild inhibitory effect on the potentiation of electroacupuncture analgesia caused by stimulation of SON, V1 antagonist has no obvious effect, oxytocin antagonist can completely block this effect, based on the above results were discussed.