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目的:比较黑龙江凤凰山地带不同海拔及不同采收期辽东楤木叶中三萜皂苷3-O-[β-D-吡喃葡萄糖基(1→2)][β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖基-刺囊酸-28-O-β-D-吡喃葡萄糖酯(简写为ECA)和3-O-β-D-吡喃葡萄糖基-(1→3)-β-D-吡喃葡萄糖基-(1→3)-β-D-吡喃葡萄糖基-齐墩果酸-28-O-β-D-吡喃葡萄糖酯(以下简称为OLA)的含量,了解这一区域采收辽东楤木叶的最佳时间和海拔高度。方法:用反相高效液相色谱-蒸发光散射检测(HPLC-ELSD)法测试,色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm),柱温30℃;流动相为乙腈、水二元梯度系统;进样量10μL,流速1.0 m L·min~(-1);载气流速3.2 L·min~(-1);漂移管温度110℃。结果:两种三萜皂苷在各自测定的范围内均呈良好线性关系(r均为0.999 8),平均加样回收率分别为97.71%,98.06%。各批次供试品中OLA的含量都远高于ECA。从6月初至9月初,两种皂苷含量几乎都呈递增趋势,6月至8月增幅很大,差别显著,8月至9月增幅较小,甚至略减;不同海拔高度条件下同时期的叶中两种皂苷含量没有显著性差异。结论:所用测试方法简单、快捷、重复性好,可为凤凰山地带辽东楤木叶的质量控制提供依据。
Objective: To compare the effects of triterpene saponin 3-O- [β-D-glucopyranosyl (1 → 2)] [β-D-glucopyranosyl - (1 → 3)] - β-D-glucopyranosyl-echinocystic acid-28-O-β-D-glucopyranosyl ester (abbreviated as ECA) and 3-O-β-D-glucopyranose (1 → 3) -β-D-glucopyranosyl- (1 → 3) -β-D-glucopyranosyl-oleanolic acid -28-O-β-D-glucopyranosyl ester Hereinafter referred to as OLA) content, to understand the best time and altitude in the region to harvest alder leaves. Methods: The HPLC-ELSD method was used for the determination of Diamonsil C18 (4.6 mm × 250 mm, 5 μm) with a column temperature of 30 ℃. The mobile phase was acetonitrile and water binary Gradient system; injection volume 10μL, flow rate 1.0 m L · min -1; carrier gas flow rate 3.2 L · min -1; drift tube temperature 110 ℃. Results: The two triterpenoid saponins showed a good linear relationship (r = 0.999 8) within the range of their determination. The average recoveries were 97.71% and 98.06%, respectively. The content of OLA in each batch of samples is much higher than that of ECA. From the beginning of June to the beginning of September, the contents of the two saponins showed an increasing trend, with a significant increase from June to August, with a significant difference, with a small increase or even a slight decrease from August to September. In the same period at different altitudes Leaf saponin content of two no significant difference. Conclusion: The test method used is simple, fast and reproducible. It can provide the basis for the quality control of Alder leaves of Liaodong in Fenghuang Mountain area.