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目的激光诱发荧光（ＬＩＦ）技术与大肠镜检查结合起来，可实时区分恶性肿瘤和非恶性肿瘤组织，准确引导活检。方法对６２例大肠肿瘤标本的ＬＩＦ光谱进行分析。结果５６例标本恶性肿瘤主、次峰及波谷峰强度明显低于对照组织，其瘤／对照峰强度比值分别为１∶３．３６、１∶５．２２和１∶５．９４（Ｐ＜０．０５）；与非恶性肿瘤组织比较，恶性肿瘤组织主峰波长略偏红光侧，次峰略偏紫光侧，主、次峰间的波变化速率相对较缓；６例未表现出上述特点，１例为腺瘤样增生，２例结肠癌细胞间有大量纤维组织，另３例原因不能确定，推测可能与粘膜下结构有关。ＬＩＦ诊断大肠恶性肿瘤，敏感度为９０．３％，特异度为１００％，阳性预示值９８．２％，阴性预示值８３．３％，准确度为９１．９％。结论ＬＩＦ引导内镜活检是可行的，应有良好前景。
Objective Laser-induced fluorescence (LIF) technology combined with colonoscopy allows real-time differentiation between malignant and non-malignant tumors and accurate biopsy. Methods LIF spectra of 62 colorectal tumors were analyzed. Results The intensity of primary, secondary and trough peaks in 56 specimens of malignant tumors was significantly lower than that in the control tissue. The intensity ratio of the tumor/control peak was 1:3.36, 1:5.22, and 1:5.94, respectively (P<0. .05); Compared with non-malignant tumors, the main peak wavelength of malignant tumor tissue is slightly on the red side, the secondary peak is slightly on the violet side, and the wave velocity between the primary and secondary peaks is relatively slow; 6 cases do not show the above characteristics. One case was an adenomatous hyperplasia. There were a large number of fibrous tissues between colon cancer cells in 2 cases. The other 3 cases could not be identified. It is speculated that this may be related to submucosal structures. LIF was diagnosed as colorectal malignancy with a sensitivity of 90.3%, a specificity of 100%, a positive predictive value of 98.2%, a negative predictive value of 83.3%, and an accuracy of 91.9%. Conclusion LIF-guided endoscopic biopsy is feasible and should have good prospects.