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背景与目的nm23-H1基因已被证明是一个肿瘤转移抑制基因,但其相关作用机理仍不明确。本研究通过比较nm23-H1基因转染前后人高转移大细胞肺癌细胞株L9981生物学行为的变化,探讨nm23-H1基因影响人高转移大细胞肺癌细胞株生物学行为的可能机理。方法应用细胞体外增殖活性检测(MTT法)和体外侵袭力检测(改良Boyden小室法)技术分别检测nm23-H1基因转染前后人高转移大细胞肺癌细胞株L9981、L9981-pLXSN和L9981-nm23-H1肺癌细胞株体外增殖活性和侵袭力的变化;同时加入PKC特异抑制剂Calphostin C作用后,再次观察三株细胞株抑制剂作用前后细胞侵袭力、增殖力的变化。结果nm23-H1基因缺失的人高转移大细胞肺癌细胞株L9981和L9981-pLXSN体外侵袭力和增殖活性明显高于转染nm23-H1基因的L9981-nm23-H1肺癌细胞株(P<0.001);L9981和L9981-pLXSN细胞间体外侵袭力和增殖活性则无统计学差异(P>0.05)。用PKC抑制剂Calphostin C处理L9981、L9981-pLXSN和L9981-nm23-H1肺癌细胞株后,细胞体外侵袭力和增殖活性下降(P<0.001),而转染nm23-H1基因的L9981-nm23-H1细胞体外侵袭力和增殖活性仍低于L9981和L9981-pLXSN(P<0.001),L9981和L9981-pLXSN细胞间则无统计学差异(P>0.05)。结论nm23-H1基因可明显抑制L9981肺癌细胞株的细胞增殖力和侵袭力。nm23-H1基因对人高转移大细胞肺癌细胞株L9981的生物学行为的影响可能与其抑制PKC信号传导有关。
Background and objective The nm23-H1 gene has been shown to be a tumor metastasis suppressor gene, but its mechanism of action is still unclear. This study compared the biological behavior of human high metastatic large cell lung cancer cell line L9981 before and after transfection with nm23-H1 gene and explored the possible mechanism of nm23-H1 gene affecting the biological behavior of human high metastatic large cell lung cancer cell line. Methods MTT assay and in vitro invasive assay (Boyden chamber assay) were used to detect the expression of L9981, L9981-pLXSN and L9981-nm23-nm23 in human highly metastatic large cell lung cancer cell line before and after transfection with nm23- H1 lung cancer cell lines in vitro proliferation activity and invasiveness changes; while adding PKC specific inhibitor Calphostin C role again observed three cell lines before and after inhibition of cell invasiveness and proliferation changes. Results The invasiveness and proliferation of L9981 and L9981-pLXSN cells with nm23-H1 gene deletion were significantly higher than those of L9981-nm23-H1 lung cancer cell line transfected with nm23-H1 gene (P <0.001). There was no significant difference in invasiveness and proliferation between L9981 and L9981-pLXSN cells in vitro (P> 0.05). In vitro, the invasion and proliferation of L9981, L9981-pLXSN and L9981-nm23-H1 lung cancer cell lines were inhibited by PKC inhibitor Calphostin C (P <0.001), while the expression of L9981-nm23-H1 The in vitro invasiveness and proliferation activity of L9981 and L9981-pLXSN were still lower than those of L9981 and L9981-pLXSN (P <0.001). There was no significant difference between L9981 and L9981-pLXSN cells (P> 0.05). Conclusion The nm23-H1 gene can significantly inhibit the cell proliferation and invasiveness of L9981 lung cancer cell line. The effect of nm23-H1 gene on the biological behavior of human highly metastatic large cell lung cancer cell line L9981 may be related to its inhibition of PKC signaling.