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目的 :研究探讨喉鳞癌发生、发展中相关基因群的表达和初步功能。 方法 :按微矩阵排列的 40 96种全长基因 PCR产物制成 Bio Door40 96型微矩阵表达谱芯片 ;采用条件优化的一步法抽提喉鳞癌及正常组织总 RNA,用 Qiagen公司 Oligotexm RNA离心柱分离纯化两种组织的 m RNA;经逆转录合成荧光分子 (Cy3/ Cy5 )掺入的 c DNA一链制备表达谱探针 ,芯片杂交和严格洗片后 ,用 Scan Array30 0 0荧光扫描仪扫描芯片荧光信号图像 ,利用计算机分析肿瘤及正常组织中差异表达的基因。对所获得的基因进行分子生物信息学分析。结果 :在 40 96种基因中 ,喉鳞状细胞癌与正常组织间存在差异表达的基因。在所检测的 4对临床标本中 ,发现有差异表达的基因 36条 (0 .88% )。生物信息学分析显示 ,该 36条差异表达基因与肿瘤的发病机制可能存在相关性。结论 :喉鳞状细胞癌的发生、发展中存在多基因表达调控的改变 ,对于相关基因群的研究有助于认识肿瘤发病机制
Objective: To study the expression and primary function of related gene cluster in the occurrence and development of laryngeal squamous cell carcinoma. Methods: 40 96 full-length cDNA microarray microarray microarray microarray microarray was used to prepare micro-matrix expression microarray chip. Total RNA was extracted from laryngeal squamous cell carcinoma and normal tissues by one-step optimization. Qiagen Oligotexm RNA was centrifuged The m RNA of both tissues was isolated and purified. The expression profile probe was prepared by reverse transcription synthesis of a c DNA strand doped with fluorescent molecule (Cy3 / Cy5). After hybridization and stringent washing, the cells were scanned with Scan Array30 0 fluorescence scanner The fluorescence signal of the chip was scanned and the differentially expressed genes in tumors and normal tissues were analyzed by computer. The obtained genes were analyzed by molecular bioinformatics. RESULTS: Of the 40 96 genes, differentially expressed genes were present between laryngeal squamous cell carcinoma and normal tissues. Among the four pairs of clinical samples tested, 36 differentially expressed genes (0.88%) were found. Bioinformatics analysis showed that the 36 differentially expressed genes may be related to the pathogenesis of tumors. Conclusions: The occurrence and development of laryngeal squamous cell carcinoma have the change of multi-gene expression and regulation, and the research on related gene groups is helpful to understand the pathogenesis of tumor