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目的观察胸腺肽α1(thymosin α1,Tα1)体外刺激培养胃癌患者外周血单个核细胞(peripheral blood mono-nuclear cells,PBMCs)对淋巴细胞亚群分化及分泌细胞因子谱的影响,分析Tα1对肿瘤患者免疫功能的影响。方法分离32例胃癌患者及18例健康自愿者PBMCs,50、10、1μg/mlTα1体外刺激培养2d;流式细胞仪检测CD4+、CD8+、CD4+CD25+Foxp3+T细胞百分比、CD4+/CD8+变化及分泌Th1/Th2细胞因子谱变化。结果 Tα1体外刺激PBMCs后,胃癌患者及健康自愿者的CD4+、CD8+T细胞水平及CD4+/CD8+比值均无明显变化;健康自愿者CD4+CD25+Foxp3+T细胞水平无明显变化,胃癌患者CD4+CD25+Foxp3+T细胞水平明显提高(P<0.05);1μg/mlTα1促进胃癌患者PBMCs分泌IL-1β、TNF-α,10μg/ml促进胃癌患者PBMCs分泌IL-6(P<0.05),健康自愿者PBMCs分泌细胞因子谱变化不明显(P>0.05)。结论 Tα1体外刺激胃癌患者PBMCs,提高CD4+CD25+Foxp3+Treg细胞亚群水平,可能对肿瘤患者抗肿瘤免疫起抑制效应。
Objective To investigate the effects of thymosin α1 (Tα1) on the differentiation and secretion of cytokines in peripheral blood mononuclear cells (PBMCs) from patients with gastric cancer in vitro and to analyze the effects of Tα1 on the immunity of tumor patients Effect of function. Methods PBMCs were isolated from 32 gastric cancer patients and 18 healthy volunteers. 50,10,1μg / ml Tα1 were cultured in vitro for 2 days. The percentage of CD4 +, CD8 +, CD4 + CD25 + Foxp3 + T cells and CD4 + / CD8 + T cells were measured by flow cytometry Secretion of Th1 / Th2 cytokine profile changes. Results There was no significant change in CD4 +, CD8 + T cells and CD4 + / CD8 + ratio between gastric cancer patients and healthy volunteers after Tα1 stimulated PBMCs in vitro. CD4 + CD25 + Foxp3 + T cells in healthy volunteers had no significant changes. CD4 (P <0.05). IL-1β, TNF-α and IL-6 secreted by PBMCs secreted by 1μg / ml Tα1 could promote the secretion of IL-6 (P <0.05) The cytokine profile of PBMCs in volunteers did not change significantly (P> 0.05). Conclusion Tα1 stimulates PBMCs in gastric cancer patients in vitro and increases the level of CD4 + CD25 + Foxp3 + Treg cells, which may inhibit the anti-tumor immunity of tumor patients.