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沙眼衣原体（ＣＴ）及解脲脲原体（ＵＵ）是非淋菌性尿道炎中的两种主要病原体，其对生殖道的危害及引起的不孕不育已被国内外学者广泛关注。我们对３４例非淋菌性尿道炎患者分别以精液及尿道拭子为标本，以ＰＣＲ技术检测，结果精液标本中ＣＴ及ＵＵ检出的总阳性率为６７．６％（２３／３４），同时检出滴虫５例，霉菌２例。而尿道拭子标本总阳性率为３５．２９％（１２／３４）。未检出滴虫及霉菌，我们认为以精液为标本，取材方便，阳性检出率高。
Chlamydia trachomatis (CT) and Ureaplasma urealyticum (UU) are the two main pathogens in non-gonococcal urethritis. The harm to reproductive tract and infertility caused by Ureaplasma urealyticum have been widely noticed by domestic and foreign scholars. In 34 cases of non-gonococcal urethritis patients were semen and urethral swab samples were detected by PCR, the results of semen specimens CT and UU positive rate was 67.6% (23/34), at the same time Trichomonas were detected in 5 cases, 2 cases of mold. The total positive rate of urethral swab specimens was 35.29% (12/34). Trichomonas and mold were not detected, we believe that the semen as samples, drawn convenient, positive detection rate.