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从单一人份HBsAg(adr)携带者血清中分离纯化HBV DNA,将其插在质粒pBR 322DNA的BamHI切点,转化大肠杆菌。经筛选得到了含完整乙型肝炎病毒(HBV)基因组的分子克隆,确定了8种限制性内切酶的切点数和位置,并用已知DNA序列的adw_2亚型HBV DNA基因特异性探针进行了基因定位。
HBV DNA was isolated and purified from the serum of a single human HBsAg (adr) carrier and inserted into the BamHI site of plasmid pBR 322 DNA for transformation into E. coli. The molecular clones containing the complete genome of hepatitis B virus (HBV) were screened, the number of cut points and positions of the eight restriction enzymes were determined and the HBV DNA gene-specific probes of the adw_2 subtype of the known DNA sequence Gene mapping.