【摘 要】
：
Granulocytes and plasmatocytes play important roles in clearing foreign ob-jects in insects,but it is difficult to distinguish between them in immune reactions.Based on the hemocyte cell line SYSU-OfHe-C established at our lab,two cell sublines,SYSU-OfHe-
【机 构】
：
School of Agriculture,Sun Yat-Sen University,Guangzhou,510275,China;State key Laboratory of Biocontr
论文部分内容阅读
Granulocytes and plasmatocytes play important roles in clearing foreign ob-jects in insects,but it is difficult to distinguish between them in immune reactions.Based on the hemocyte cell line SYSU-OfHe-C established at our lab,two cell sublines,SYSU-OfHe-C Granulocyte (Gr cells) and SYSU-OfHe-C Plasmatocyte (Pl cells),which pos-sess the morphological characteristics of granulocytes and plasmatocytes,respectively,were established.Gr and Pl cells showed different behaviors in immune reactions,such as spreading,phagocytosis and encapsulation.Pl cells were easier to spread,but Gr cells tended to undergo aggregation,indicating that they may take different strategies to clear foreign objects.These results also suggested that granulocytes and plasmatocytes may ex-press some different proteins.By comparing the gene expression in cells from the two sublines,1662 differentially expressed genes were identified,and 13 out of 30 transmem-brane proteins highly expressed in P1 cells (six) or Gr cells (seven) were further screened and confirmed by reverse-transcription polymerase chain reaction (PCR).Finally,three transmembrane genes specifically expressed in P1 cells and two transmembrane genes specifically expressed in Gr cells were screened out based on their expressions in immune reactions by quantitative PCR analysis.These genes may potentially be used as molecu-lar markers to distinguish between granulocytes and plasmatocytes in Ostriniafurnacalis,and further to clarify the functions of immune hemocytes in cellular immune reaction such as encapsulation and so on.
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