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The chromatin folding pattes in air and liquid were studied by atomic force microscopy (AFM). A gentle water-air interface method was adopted to spreadchromatin from interphase nucleus of chicken erythrocyte. Thechromatin was absorbed on APS-mica surface and studied with AFM.Beads-on-a-string were observed and many higher-order structures suchas superbeads with dimensions 40～60 nm in diameter and 4～7 nmin height were found to string together to make chromatin fibers. Whensample spreading and absorbing time were shortened, higher-orderchromatin fibers with 60～120 nm in width were observed in air as wellas under water environment. These chromatin structures may reflectchromatin folding pattes in the living cells.