论文部分内容阅读
目的:建立HPLC法同时测定了哥王中芦荟大黄素和大黄素甲醚含量的方法。方法:采用C18色谱柱(4.6 mm×250 mm,5μm),流动相A为乙腈,流动相B为体积分数为0.15%磷酸水,梯度洗脱,流速1.0 m L·min-1,检测波长254 nm,柱温30℃。结果:芦荟大黄素和大黄素甲醚的质量浓度分别在1.584~25.34(r=0.999 2)和1.598~25.57μg·m L-1(r=0.999 3)内与峰面积呈良好的线性关系,平均回收率分别为100.2%(RSD 1.8%)和99.7%(RSD 1.1%)。不同产地了哥王中大黄素甲醚和芦荟大黄素含量差异较大,其中广东韶关产地的芦荟大黄素含量最高,大黄素甲醚含量最低;福建福州产地的大黄素甲醚含量最高,芦荟大黄素含量最低;浙江(批号120724)了哥王未检测到芦荟大黄素,贵州安顺产地的了哥王中未检测到大黄素甲醚。结论:该方法简便、快速、准确,为了哥王药材的质量控制提供参考。
OBJECTIVE: To establish a HPLC method for simultaneous determination of aloe-emodin and physcion in Columbia. Methods: C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase A was acetonitrile and the mobile phase B was 0.15% phosphoric acid with a gradient of 1.0 mL · min-1. The detection wavelength was 254 nm, column temperature 30 ℃. Results: The mass concentration of aloe-emodin and physcion was linear with the peak area in 1.584-25.34 (r = 0.999 2) and 1.598-25.57 μg · m L-1 (r = 0.999 3) The average recoveries were 100.2% (RSD 1.8%) and 99.7% (RSD 1.1%), respectively. In different areas, the contents of emodin and aloe-emodin in Brother King varied greatly, among them, the content of aloe-emodin in Shaoguan origin was the highest and the content of physcion was the lowest. The content of emodin in the origin of Fuzhou was the highest, The lowest content of vegetarian; Zhejiang (batch number 120724) Columbia King did not detect aloe-emodin, Guizhou Anshun origin of Columbia King was not detected in the ether. Conclusion: The method is simple, rapid and accurate, and provides a reference for the quality control of Gordian herbs.