论文部分内容阅读
采用ＤＯＣＡ硅胶管皮下埋入法建立大鼠心衰模型，从基因转录水平检测正常与心衰大鼠心肌组织中心肌收缩蛋白分子基因α－ＭＨＣ、β－ＭＨＣ、α－ｃａｒｄｉａｃａｃｔｉｎ、α－ｓｋｅｌｅｔａｌａｃｔｉｎ表达的变化。结果显示：（１）心衰大鼠心肌收缩力指标ｄｐ／ｄｔｍａｘ较正常大鼠明显降低（下降２７．５１％，Ｐ＜０．０１），（２）心衰大鼠与正常大鼠相比，心肌组织中α－ＭＨＣ基因表达水平显著下降（降低２１．４３％，Ｐ＜０．０５），β－ＭＨＣ基因表达水平显著升高（升高６２．４３％，Ｐ＜０．０１）、α－ｃａｒｄｉａｃａｃｔｉｎ基因和α－ｓｋｅｌｅｔａｌａｃｔｉｎ基因表达水平未见明显改变，（３）α－ＭＨＣｍＲＮＡ的含量与心肌收缩力指标ｄｐ／ｄｔｍａｘ值之间存在正相关关系（ｒ＝０．４１４３，ｎ＝４３，Ｐ＜０．０５），β－ＭＨＣｍＲＮＡ的含量与ｄｐ／ｄｔｍａｘ值之间存在负相关关系。（ｒ＝－０．３９０２，ｎ＝４３，Ｐ＜０．０５）。这提示：心肌组织中ＭＨＣ基因表达水平的改变是心衰时心肌收缩力降低的主要分子基础
The rat model of heart failure was established by subcutaneous burying of DOCA silicone tube. The expression of α-MHC, β-MHC, α-cardiacactin and α-skeletalbumin in myocardium of normal and heart failure rats were detected by gene transcription The change. The results showed that: (1) The dp / dtmax of myocardial contractility in heart failure rats was significantly lower than that in normal rats (27.51%, P <0.01); (2) Compared with normal rats The level of α-MHC gene expression was significantly decreased (21.43%, P <0.05), the level of β-MHC gene expression was significantly increased (62.43%, P <0.01) α-cardiacactin and α-skeletalactin showed no significant changes. (3) There was a positive correlation between α-MHC mRNA and dp / dtmax (r = 0.4143, n = 43, P <0.05). There was a negative correlation between the content of β-MHC mRNA and dp / dtmax. (R = -0.3902, n = 43, P <0.05). This suggests that changes in MHC gene expression in cardiac tissue are the major molecular basis for decreased cardiac contractility during heart failure