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目的:通过本实验室试验结果以及参考前人的文献资料,浅谈虎红平板凝集试验(RBPT)以及试管凝集试验(SAT)两种检测方法检测布鲁氏菌病的优缺点。方法:用虎红平板凝集试验(RBPT)以及试管凝集试验(SAT)分别对患者血清进行布鲁氏菌病检测,对检测结果进行分析。结果:RBPT相对于SAT,因试验时间、环境温度等影响,存在一定的假阳性,以及少量的假阴性结果。结论:大范围调查检测宜先用RBPT进行初筛,对阳性者用SAT确诊;对于少量的样本检测,可直接用SAT进行确诊。
OBJECTIVE: To evaluate the advantages and disadvantages of brucellosis by RBPT and SAT, based on the results of our laboratory and the references of previous literatures. Methods: Brucellosis was detected in patients’ serum by RBPT and SAT respectively. The test results were analyzed. Results: RBPT relative to the SAT, due to the impact of test time, ambient temperature, there are some false positives, as well as a small amount of false negative results. CONCLUSIONS: A wide range of screening tests should be performed initially with RBPT, positive with SAT, and with a small sample size with SAT.