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人们已使用了许多将蛋白质交联至红细胞膜上作为被动血凝(PHA)试剂的方法.其原理不外乎两种,(1)用醛、磺基水杨酸或鞣酸“固定”红细胞,接着通过吸附或用化学交联剂将蛋白质交联于细胞表面,(2)用不同双功能或多功能试剂对蛋白质进行共价交联.虽然这些技术被广泛应用,但仍然存在不同程度的缺陷.大多数交联步骤中,细胞表面被改变,导致特异性致敏的红细胞上非特异性地吸附血清蛋白,此非特异性本底影响了PHA方法的灵敏性和特异性.本文作者介绍了一种条件温和,没
There are many ways in which proteins can be cross-linked to the erythrocyte membrane as passive hemagglutination (PHA) reagents, and there are no more than two principles: (1) aldehydes, sulfosalicylates, or tannic acid "Red blood cells, which are then cross-linked by adsorption or chemical cross-linking on the cell surface, (2) covalent cross-linking of proteins with different bifunctional or multifunctional reagents, and while these technologies are widely used, there are still differences Degree of defects in most of the cross-linking step, the cell surface is changed, resulting in specific sensitized erythrocyte nonspecific adsorption of serum proteins, this non-specific background has affected the sensitivity and specificity of the PHA method. A mild condition, no