,Separation and simultaneous quantitation of PGF2α and its epimer 8-iso-PGF2α using modifier-assiste

• 作 者: Chunsu Liang Hui Sun Xiangjun Meng Lei Yin J.Paul Fawcett Huaidong Yu Ting Liu Jingkai Gu
• 机 构: Research Center for Drug Metabolism,School of Life Sciences,Jilin University,2699 Qianjin Street,Cha
• 出 处: 药学学报（英文版）
• 发表日期: 2018年2期
• 关键词: Differential mobility spectrometry Mass spectrometry Epimer PGF2α 8-iso-PGF2α

Because many therapeutic agents are contaminated by epimeric impurities or form epimers as a result of metabolism,analytical tools capable of determining epimers are increasingly in demand.This article is a proof-of-principle report of a novel DMS-MS/MS method to separate and simultaneously quantify epimers,taking PGF2α and its 8-epimer,8-iso-PGF2α,as an example.Good accuracy and precision were achieved in the range of 10-500 ng/mL with a run time of only 1.5 min.Isopropanol as organic modifier facilitated a good combination of sensitivity and separation.The method is the first example of the quantitation of epimers without chromatographic separation.