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目的研究波棱瓜愈伤组织诱导、分化及生根的条件,探讨波棱瓜快速繁殖新途径,为波棱瓜组织培养与离体植株再生奠定基础。方法以波棱瓜幼嫩茎段和叶片为材料,比较不同外植体、基本培养基及植物生长调节剂对波棱瓜愈伤组织诱导、分化及生根的影响。结果幼嫩茎段作为外植体时,其出愈率较叶片高,愈伤量大;MS基本培养基能够产生明显的愈伤组织,出愈率较高;幼嫩茎段愈伤组织诱导适宜的培养基为MS+6-BA2 mg/L+NAA0.5 mg/L,出愈率达93.3%。愈伤组织的最佳分化培养基为MS+6-BA2 mg/L+NAA0.1 mg/L+KT 3.0 mg/L,分化率达87.2%。生根培养的适宜培养基为1/2MS+NAA 1.0 mg/L,生根率达95%。结论波棱瓜幼嫩茎段在适宜的培养基中可通过愈伤组织途径分化不定芽,且极易生根,能够得到正常生长发育的再生植株。
OBJECTIVE To study the conditions of callus induction, differentiation and rooting in Boleoglitazone, to explore a new way of rapid propagation of Bongogua melon so as to lay a foundation for the tissue culture of Bole County and in vitro plant regeneration. Methods The young stems and leaves of Boleogarden were used as materials to compare the effects of different explants, basal medium and plant growth regulators on the induction, differentiation and rooting of Bruggeissue melon. Results When young stem segments were used as explants, the emergence rate was higher than that of leaves and callus. MS basic medium could produce obvious callus, and the callus was higher. The young stem callus induction The suitable culture medium was MS + 6-BA2 mg / L + NAA0.5 mg / L, with a cure rate of 93.3%. The best differentiation medium for callus was MS + 6-BA2 mg / L + NAA0.1 mg / L + KT 3.0 mg / L with a differentiation rate of 87.2%. The suitable culture medium for rooting was 1 / 2MS + NAA 1.0 mg / L with the rooting rate of 95%. Conclusion The young stems of Boleogua melon can differentiate into adventitious buds through callus in suitable culture medium, and they are easily rooted and regenerated plants with normal growth and development can be obtained.