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目的观察新生Wistar鼠脑缺血预处理后海马CA1区突触素(Syp)和胶原纤维酸性蛋白(GFAP)的动态变化。方法阻断7日龄新生Wistar大鼠右侧颈总动脉制备脑缺血模型,设置假手术组、缺血再灌注组、预缺血-缺血再灌注组。通过免疫组化方法检测3组新生鼠不同时点海马CA1区脑组织神经细胞Syp和GFAP的动态变化并观察其病理改变。结果缺血再灌注组和预缺血-缺血再灌注组海马CA1区Syp表达在再灌注后第3天开始增加,7d达高峰,14d仍高于对照组,3组比较差异有显著性;缺血再灌注组和预缺血组海马CA1区GFAP表达在再灌注后24h开始增高,3～14d持续在较高水平,3组比较差异有显著性;预缺血-缺血再灌注组病理改变较缺血再灌注组轻。结论新生鼠脑缺血后脑神经细胞具有代偿和修复的可塑性;经脑缺血预处理后,对再次脑缺血所致脑神经细胞损伤有保护作用;反应性星形胶质细胞增生在缺血耐受中可能起重要作用。
Objective To observe the dynamic changes of synaptophysin (SypA) and fibrillary acidic protein (GFAP) in hippocampal CA1 after cerebral ischemic preconditioning in neonatal Wistar rats. Methods The right common carotid artery of 7-day-old Wistar rats was induced to establish cerebral ischemia model. Sham-operation group, ischemia-reperfusion group and pre-ischemia-reperfusion group were established. Immunohistochemistry was used to detect the dynamic changes of Syp and GFAP in the hippocampal CA1 subfields in 3 groups of neonatal rats at different time points and their pathological changes were observed. Results The expression of Syp in hippocampal CA1 subfields in ischemia - reperfusion group and preperfusion - ischemia reperfusion group increased from the third day after reperfusion, reaching the peak on the 7th day and still higher than the control group on the 14th day. There was significant difference among the three groups. The expression of GFAP in hippocampal CA1 area of ischemic reperfusion group and preconditioned group began to increase at 24h after reperfusion, and remained high at 3 ~ 14d, the difference was significant among the three groups; the pathological changes in pre-ischemia-reperfusion group Change than ischemia-reperfusion group light. Conclusion Neonatal rat cerebral neurons after cerebral ischemia have compensatory and repair plasticity. After cerebral ischemic preconditioning, they have a protective effect on cerebral nerve cell injury caused by re-ischemic cerebral ischemia. Reactive astrocytes proliferate in the absence Blood tolerance may play an important role.