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利用gTME方法获得高效利用木糖并共发酵木糖和葡萄糖的重组酿酒酵母菌株YPH499-3。以玉米芯为原料,在120℃、固液比为1∶10(m/v)、3%HCl处理2h,糖的总质量浓度达到43.8g/L,木糖和葡萄糖质量浓度分别为38.95g/L和4.85g/L,gTME重组菌株YPH499-3与出发菌株YPH499在不同起始pH浓缩玉米芯水解液中进行厌氧发酵,结果显示:YPH499-3能在广泛的不同起始pH的玉米芯水解液中生长良好。在起始pH为5、处理71h时,YPH499-3最大木糖和葡萄糖利用率分别为65.7%和86.6%,乙醇最大质量浓度为8.90g/L;出发菌株YPH499葡萄糖利用率最大值67.4%,不利用木糖,乙醇最大质量浓度为2.38g/L。
The gTME method was used to obtain a recombinant Saccharomyces cerevisiae strain YPH499-3 that efficiently utilizes xylose and co-ferments xylose and glucose. Using corn cob as raw material, the total mass concentration of sugar reached 43.8g / L and the mass concentration of xylose and glucose were respectively 38.95g and 120g at 120 ℃ with solid-liquid ratio of 1:10 (m / v) and 3% HCl for 2h. / L and 4.85g / L respectively, the gTME recombinant strain YPH499-3 and the starting strain YPH499 were anaerobically fermented in different initial pH-concentrated corncob hydrolyzate. The results showed that YPH499-3 could be used in a wide range of corn with different initial pH The core hydrolyzate grows well. The maximal xylose and glucose utilization rates of YPH499-3 were 65.7% and 86.6% at the initial pH of 5 and 71 h, respectively. The maximum concentration of ethanol was 8.90 g / L. The highest glucose utilization rate of YPH499 was 67.4% Do not use xylose, the maximum concentration of ethanol 2.38g / L.