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目的建立大鼠血清丹参素的高效液相分析法,测定不同量口服给予抗纤二号血清中丹参素的含量。方法大鼠4d 7次给药(0.45 g/次或0.55 g/次,2次/d),于不同时间心内取血用HPLC测定丹参素含量;HPLC条件:色谱柱polairsC18-A 4.6×150 mmΦ5μm,流动相乙腈-磷酸盐缓冲液(5:95),紫外检测波长205 nm。结果连续给药均在给药1 h给药后达到最高血药浓度,大剂量药物血清血药浓度高于中剂量药物血清.丹参素在76~1520 mg/L范围内呈线性关系(r=0.9932)。加样回收率在76 mg/L、304 mg/L、1520 mg/L时分别为98.3%、101.5%、99.3%,相对标准偏差(RSD)分别为5.9%、2.5%、2.0%。以上间接证明该中药复方动物给药能达到有效的血药浓度。结论大剂量药物血清中丹参素的血药浓度高于中剂量药物血清。
Objective To establish a HPLC method for the determination of Danshensu in rat serum and determine the content of Danshensu in oral anti-fibrosis II serum. Methods Rats were given 4 days (0.45 g/time or 0.55 g/time, twice times daily). Blood samples were taken from the heart at different time to determine the content of danshensu by HPLC; HPLC conditions: column polairsC18-A 4.6×150 mmΦ5μm, mobile phase acetonitrile-phosphate buffer (5:95), UV detection wavelength 205 nm. Results The highest plasma concentration was reached after continuous administration for 1 h after administration. The plasma concentration of high-dose drug was higher than that of middle-dose drug serum. Danshensu was linear in the range of 76 to 1520 mg/L (r= 0.9932). The recoveries of the samples at the spiked levels of 76 mg/L, 304 mg/L, and 1520 mg/L were 98.3%, 101.5%, and 99.3%, respectively, and the relative standard deviations (RSDs) were 5.9%, 2.5%, and 2.0%, respectively. The above indirect proof of the traditional Chinese medicine compound animal administration can achieve effective blood drug concentration. Conclusion The plasma concentration of danshensu in high-dose drug serum is higher than that of medium-dose drug serum.