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目的探讨宫颈癌发生发展过程中,表皮生长因子受体(EGFR)与人乳头瘤病毒(HPV)的作用及其相互关系。方法宫颈癌组60例,选自1997年至2001年间中山大学肿瘤防治中心住院初治的宫颈癌病例,临床分期Ⅰa～Ⅱb期;宫颈上皮内瘤变(CIN)组40例;正常上皮对照组30例。以免疫组化S-P法检测宫颈组织EGFR的表达,以PCR检测HPV16和(或)HPV18感染。结果正常上皮组、CIN组和宫颈癌组的EGFR中强表达率呈梯度上升,分别为0、42.5%和76.7%,差异有统计学意义(P<0.05)。正常上皮组、CIN组和宫颈癌组的HPV16和(或)HPV18感染率分别为6.7%、67.5%和58.3%,宫颈癌组和CIN组的感染率均显著高于正常上皮组(P=0.000),但宫颈癌组与CIN组之间,差异无统计学意义(P=0.355)。肿瘤侵袭程度超过宫颈1/2间质者,EGFR中强表达率显著高于未达1/2间质者(89.2%:56.5%,P=0.004)。宫颈管侵袭者HPV16和(或)HPV18感染率显著高于无侵袭者(88.2%:46.5%,P=0.003)。EGFR与HPV之间无显著相关性(P>0.05)。EGFR与HPV均未显示与宫颈癌预后有关。结论EGFR和HPV与宫颈癌的发生发展有关;EGFR、HPV16和(或)HPV18与宫颈癌预后无关,EGFR与HPV16和(或)HPV18无显著相关性。
Objective To investigate the role and relationship between epidermal growth factor receptor (EGFR) and human papillomavirus (HPV) in the development and progression of cervical cancer. Methods 60 cases of cervical cancer group were selected from hospitalized patients with cervical cancer who were admitted to Sun Yat-sen University Cancer Center from 1997 to 2001. The patients were divided into two groups: Ⅰa-Ⅱb stage, 40 cases of cervical intraepithelial neoplasia (CIN) group, 30 cases. The expression of EGFR in cervical tissue was detected by immunohistochemical S-P method, and HPV16 and / or HPV18 infection was detected by PCR. Results The strong positive rate of EGFR in normal epithelium, CIN group and cervical cancer group showed a gradient increase of 0, 42.5% and 76.7%, respectively, with statistical significance (P <0.05). The infection rates of HPV16 and HPV18 in normal epithelium group, CIN group and cervical cancer group were 6.7%, 67.5% and 58.3%, respectively. The infection rates in cervical cancer group and CIN group were significantly higher than those in normal epithelium group (P = 0.000 ), But there was no significant difference between cervical cancer group and CIN group (P = 0.355). The rate of tumor invasion was higher than that of cervix 1/2, and the strong expression rate in EGFR was significantly higher than that in less than 1/2 (89.2%, 56.5%, P = 0.004). Cervical invasion of HPV16 and / or HPV18 infection rates were significantly higher than non-invasive (88.2%: 46.5%, P = 0.003). There was no significant correlation between EGFR and HPV (P> 0.05). Both EGFR and HPV showed no correlation with the prognosis of cervical cancer. Conclusions EGFR and HPV are related to the occurrence and development of cervical cancer. EGFR, HPV16 and / or HPV18 have no relationship with the prognosis of cervical cancer, and there is no significant correlation between EGFR and HPV16 and / or HPV18.