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目的探讨银杏苦内酯B(BN52021)对创伤性脑损伤大鼠脑组织病理形态学的影响。方法 48只SD大鼠随机分为4组:假手术组、模型组、低剂量BN52021组和高剂量BN52021组,每组12只。后3组制作液压打击脑损伤模型。低剂量BN52021组和高剂量BN52021组于模型制作当天开始分别腹腔注射1mg/kg和5mg/kg BN52021,每天1次,连续7d。第7天治疗后取脑组织进行形态学及免疫组织化学检测和超微结构观察。结果与假手术组相比,模型组大鼠脑组织神经元相对数量减少(P<0.05),OX-42阳性小胶质细胞和星形胶质细胞数量增加(P<0.05),caspase-3阳性细胞数增多(P<0.05);电镜下可见神经元染色质边集,部分出现核碎裂、核溶解,线粒体变圆变大,空泡形成,嵴消失,内质网增宽,溶酶体增多,核膜皱褶。低剂量和高剂量BN52021组与模型组比较,小胶质细胞和星形胶质细胞比例减少(P<0.05),caspase-3阳性细胞数减少(P<0.05),超微结构明显改善;高剂量组较低剂量组改善更加明显。结论 BN5202对创伤性脑损伤大鼠脑组织具有保护作用。
Objective To investigate the effect of ginkgolide B (BN52021) on pathomorphology of brain in traumatic brain injury rats. Methods Forty eight Sprague-Dawley rats were randomly divided into 4 groups: sham operation group, model group, low dose BN52021 group and high dose BN52021 group, with 12 rats in each group. The latter three groups produced a hydraulic combat brain injury model. The low dose BN52021 group and the high dose BN52021 group were intraperitoneally injected with 1 mg / kg and 5 mg / kg of BN52021 once a day for 7 days continuously on the day of model making. After 7 days of treatment, brain tissue was taken for morphological and immunohistochemical examination and ultrastructure observation. Results Compared with the sham operation group, the relative number of neurons in the model group decreased (P <0.05), the number of OX-42 positive microglial cells and astrocytes increased (P <0.05), while caspase-3 The number of positive cells increased (P <0.05). The neuronal chromatin marginal set was observed under electron microscope. Some of the neurons were nuclear fragmentation, nuclear dissolution, rounding of mitochondria became larger, vacuoles formed, cristae disappeared, endoplasmic reticulum broadened, Increased body, nuclear membrane folds. Compared with the model group, the proportion of microglia and astrocyte decreased (P <0.05), the number of caspase-3 positive cells decreased (P <0.05), the ultrastructure of BN52021 group decreased significantly The dose group improved more significantly in the lower dose group. Conclusion BN5202 has a protective effect on brain tissue in traumatic brain injury rats.