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目的：研究在体情况下ＮＯ对兔肝胰壶腹（Ｏｄｄｉ）括约肌运动的调节及其可能机制。方法：家兔麻醉剖腹后，经胆总管插入一直径为０．７ｍｍ的灌注测压导管，将测压导管开口固定于肝胰壶腹括约肌处，检测肝胰壶腹括约肌动力。结果：静脉滴注左旋硝基精氨酸甲酯（Ｌ－ＮＡＭＥ）阻断ＮＯ合成后，括约肌基础压力、收缩幅度及收缩频率均明显增加（Ｐ＜０．０５）；右旋精氨酸、阿托品、酚妥拉明及普萘洛尔（心得安）不能阻断Ｌ－ＮＡＭＥ的兴奋效应；大剂量左旋精氨酸及ＮＯ供体硝普钠则可阻断该效应。结论：ＮＯ通过非胆碱能、非肾上腺素能途径对肝胰壶腹括约肌运动起抑制作用。
OBJECTIVE: To study the regulation of NO on sphincter of rabbits with hepatobiliary and pancreatic diverticulitis in vivo and its possible mechanism. Methods: After the anesthetized cesarean section in rabbits, a catheter with a diameter of 0.7mm was inserted through the common bile duct, and the opening of the pressure-measuring catheter was fixed at the amputated sphincter of hepatopancreas. Results: After L-NAME blocked NO synthesis, the basic pressure, contractile amplitude and contractile frequency of sphincter were significantly increased (P <0.05). The concentrations of L-arginine, Atropine, phentolamine and propranolol (propranolol) can not block the excitement effect of L-NAME; high-dose L-arginine and NO donor sodium nitroprusside can block this effect. CONCLUSION: NO inhibits hepatic-pancreatic amputation in a non-cholinergic and non-adrenergic manner.