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目的研究药物诱导白血病细胞凋亡中的电化学变化及其意义。方法使用电化学传感器检测经足叶乙甙（Ｖｐ１６）诱导的ＨＬ６０细胞凋亡。结果２～２００μｇ／ｍｌＶｐ１６作用于ＨＬ６０细胞２４小时的过程中，随着药物剂量的增大，细胞峰值电流逐渐降低，Ｖｐ１６２μｇ／ｍｌ时即可观察到细胞电化学活性较对照组下降，２０μｇ／ｍｌ时明显下降。以Ｖｐ１６２００μｇ／ｍｌ诱导细胞凋亡，通过ＤＮＡ含量分析显示在２，３，４小时细胞凋亡率为１０．５％～２０．０％，用电化学传感器方法检测细胞电化学活性改变差异明显，峰值电流从１．８μＡ降至０４μＡ，４小时时峰值电流降至用药前的１／５。结论在细胞凋亡早期启动阶段的电化学信号改变具有一定的早期意义和蓄积性。
Objective To study the electrochemical changes in drug-induced leukemia cell apoptosis and its significance. Methods Electrochemical sensors were used to detect apoptosis of HL-60 cells induced by etoposide (Vp16). Results 2 ~ 200μg / ml Vp16 role in HL 60 cells for 24 hours in the process, with the increase of drug dose, cell peak current gradually decreased, Vp162μg / ml can be observed when compared with the control group cell electrochemical activity decreased 20μg / Ml significantly decreased. Cell apoptosis was induced by Vp16200μg / ml. The DNA content analysis showed that the cell apoptosis rate was 10.5% ~ 20.0% at 2, 3 and 4 hours. The change of electrochemical activity of cells was obvious with electrochemical sensor. The peak current was reduced from 1.8μA to 0.4μA, and the peak current dropped to 1/5 of that before 4 hours. Conclusion The change of electrochemical signal during the early stage of apoptosis is of some early significance and accumulation.