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质谱(MS)是表征蛋白质和蛋白质翻译后修饰(PTM)的一种强大的技术手段,在现代生物学研究领域中发挥着越来越重要的作用.近年来,非变性质谱也已成功用于研究蛋白质非共价相互作用,在研究化学计量、结构功能和蛋白质组装动力学方面彰显出灵敏、快速、专一及信息量大等优势.这种在结构生物学前沿领域应用的技术工具发展于20世纪90年代初期,虽然其早期研究主要集中在利用质谱的质量测定来获得蛋白质复合物中的化学计量等信息,但随着质谱技术和方法的进一步发展,现在已能够用于揭示蛋白质结构、亚基间连接性和蛋白质动力学等生命科学问题.本文及后续文章中将对应用非变性质谱研究非共价蛋白质复合物的概况、最适宜的离子源和质量分析器、实验中可能遇到的问题及应对策略等进行综述与讨论,并辅以相关的应用实例进行较为详细的介绍.“,”Mass spectrometry (MS) is currently playing an increasingly important role in biological research as a powerful technique to characterize proteins and protein post-translational modifications (PTMs).In recent years,native or non-denaturing mass spectrometry has been successfully used to study non-covalent interactions between protein and other molecules,making it a sensitive,quick,specific and informative technique in studies of stoichiometry,structurefunction,and protein assembly dynamics.This physiochemical tool used in the frontiers of structural biology stems from experiments performed in the early 1990s.Although its initial studies focused on the elucidation of stoichiometry by means of mass determination,developments in MS technology and methodology in the past decades have allowed researchers to address questions such as protein shape,inter-subunit association and protein dynamics.In this and following articles,basic facts,the most suitable ion sources and mass analyzers,possible problems confronted in experiments and coping strategies in using native mass spectrometry to study non-covalent protein complexes are reviewed and discussed,while a number of application examples are provided for detailed introduction on certain issues.