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识别、定位小麦叶蛋白并解析小麦叶蛋白的基因组行为特征,是小麦叶蛋白质组学基础研究的一个重要方面。本研究采用高通量蛋白分析及质谱分析和生物信息学等技术,分析了普通小麦品种阿勃整倍体与其5个缺体系的黄化苗阶段叶片(E)和抽穗期叶片(H)的叶蛋白表达差异。阿勃整倍体E中重复检测到630个蛋白,阿勃整倍体H中重复检测到520个蛋白,其中514个蛋白为E和H共有,116个蛋白仅E有,6个蛋白(862,756.1,759,A56.2,458.3,249)仅H有。同时发现,染色体1A、1B、6A、6B和6D在H期影响68个叶蛋白的表达,在E期影响38个叶蛋白的表达。值得特别强调的是,叶蛋白A56.1,一个推测的逆转座子蛋白,在1A缺体系或6A缺体系的E和H期中都完全缺失,而叶蛋白756.1,一个推测的依ATP的DNA螺旋酶II 70 kD亚基,在1A缺体系或1B缺体系或6A缺体系的H期中都完全缺失。
Identifying, locating and analyzing the wheat leaf protein genome characteristics of wheat leaf protein is an important aspect of the basic research of wheat leaf proteomics. In this study, high-throughput protein analysis and mass spectrometry and bioinformatics techniques were used to analyze the relationship between the etiolated (E) and erecta Differences in leaf protein expression. A total of 630 proteins were detected in Eperythrozoon E, and 520 proteins were detected in Euploid H. In 514 of them, E was shared with H, 116 proteins were E, 6 (862, 756.1 , 759, A56.2, 458.3, 249) H only. At the same time, it was found that chromosomes 1A, 1B, 6A, 6B and 6D affected the expression of 68 leaf proteins in H phase and 38 leaf proteins in E phase. Of note, leaf protein A56.1, a putative retrotransposon protein, is completely absent in the E and H phases of either 1A-deficient or 6A-deficient systems, whereas leaf protein 756.1, a putative ATP-helical DNA helix Enzyme II 70 kD subunit was completely deleted in the H phase of 1A-deficient system or 1B-deficient system or 6A-deficient system.