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目的:探讨弥漫性大B细胞淋巴瘤(DLBCL)的临床特征和免疫分型特征。方法:分析75例DL-BCL的临床和病理资料,制作HE染色切片,并经免疫组织化学Envision plus法染色,观察CD10、bcl-6和MuM1蛋白的表达,并进一步区分生发中心B细胞(GCB细胞)和非生发中心B细胞的分化特征。结果:75例DLBCL患者,其中结内48例,结外27例,临床分期Ⅰ-Ⅱ期患者占66%(27/41),临床分期Ⅲ-Ⅳ期患者占34%(14/41);国际预后指标(IPI)0～2分者占76%(31/41),3～5分者占24%(10/41)。单个抗原的表达率,CD10(+)11例(20%),bcl-6(+)27例(49%),MuM1(+)35例(64%);18例(33%)为GCB-DLBCL,37例(67%)为非GCB-DLBCL。结论:DLBCL显示生发中心B细胞和非生发中心B细胞分化特征,中国人群DLBCL的GCB样型显著低于非GCB样型。
Objective: To investigate the clinical features and immunophenotyping features of diffuse large B cell lymphoma (DLBCL). Methods: The clinical and pathological data of 75 cases of DL-BCL were analyzed. HE staining sections were made and stained with Envision plus immunohistochemistry to observe the expressions of CD10, bcl-6 and MuM1 protein, and to further distinguish the germinal center B cells (GCB Cells) and non-germinal center B cells. Results: Among the 75 patients with DLBCL, there were 48 cases in node and 27 cases with extranodal involvement. The clinical stage Ⅰ-Ⅱ patients accounted for 66% (27/41), and the clinical stage Ⅲ-Ⅳ patients accounted for 34% (14/41). International prognostic indicators (IPI) 0 to 2 accounted for 76% (31/41), 3 to 5 points accounted for 24% (10/41). The expression rates of single antigen were 11 cases (20%) of CD10 (+), 27 cases (49%) of bcl-6 (+) and 35 cases (64%) of MuM1 37 cases (67%) of DLBCL were non-GCB-DLBCL. CONCLUSION: DLBCL shows the characteristics of B cell differentiation in germinal center and non-germinal center. The GCB sample of DLBCL in Chinese population is significantly lower than non-GCB sample.