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目的 从亚细胞水平观察大鼠在 12 0 0 0m高度暴发性缺氧及富氧气体防护时大脑顶叶皮质组织超微结构变化 ,为高空暴发性缺氧的防护和治疗提供理论依据。 方法 将 2 4只雄性Wistar大鼠随机分为地面对照组、30 0 0m对照组、12 0 0 0m暴发性缺氧组、12 0 0 0m吸 90 %富氧气体防护组、12 0 0 0m吸 10 0 %氧气防护组、12 0 0 0m吸 5 0 %富氧气体防护组 ,每组 4只。于低压舱内暴露于 12 0 0 0m高度 30min ,然后将动物即刻处死 ,取大脑顶叶皮质层组织 ,制成切片 ,透射电镜下观察。 结果 与地面对照组相比 ,随着吸氧浓度的降低 ,实验组缺氧程度加重 ,主要表现为脑组织神经细胞、神经胶质细胞结构模糊不清 ,核及胞质内线粒体、内质网扩张 ,突触小泡减少等 ,以12 0 0 0m暴发性缺氧组及吸 5 0 %氧气防护组明显。 结论 吸 10 0 %和吸 90 %富氧气体防护效果是理想的 ,与地面对照组和 30 0 0m对照组的结果无明显差别
Objective To observe the changes of ultrastructure of parietal cortex in hyperpyrexia and oxygen-rich gas protection in rats at sub-cellular level to provide a theoretical basis for the prevention and treatment of fulminant anaphylactic hypobaric disease. Methods Twenty-four male Wistar rats were randomly divided into three groups: ground control group, control group of 30 000 m, violent hypoxia of 1200 m, protective gas of 90% oxygen-enriched gas of 120 m, 10 0% oxygen protection group, 100% oxygen absorption 50% oxygen-enriched gas protection group, 4 in each group. Exposed to a height of 12 000 meters for 30 minutes in the low-pressure cabin, and then the animals were sacrificed immediately. The parietal cortex of the brain was made into slices and observed under a transmission electron microscope. Results Compared with the ground control group, with the decrease of oxygen concentration, the degree of hypoxia increased in the experimental group, which mainly manifested the fuzzy structure of neuronal cells and glial cells, the mitochondria and endoplasmic reticulum Dilation, synaptic vesicle reduction and so on, to 12 0 0m outbreak of hypoxia and oxygen inhalation group 0% 0% protective group was significantly. Conclusion The protective effect of absorbing 100% oxygen and 90% oxygen-enriched gas is ideal, with no significant difference between the control group and the control group at 300 m