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目的 研究流体切应力对人血管内皮细胞的单核细胞趋化蛋白 1(MCP 1)表达的影响 ,探讨血流动力在动脉粥样硬化 (AS)发生早期中的作用。方法 利用平行板流动腔对血管内皮细胞施加不同切应力 ,分别采用夹心酶联免疫吸附测定 (ELISA)及逆转录 聚合酶链反应 (RT PCR)法检测MCP 1蛋白及mRNA水平。结果 以 0 .72Pa切应力进行不同时间作用 ,0 .5h后MCP 1mRNA即达到静态时 (0 16 0± 0 0 37)的 4倍 (0 .6 84± 0 .0 33) ,5h时略有升高 ,达 0 .70 7± 0 .0 89,12h后下降到低于对照组的水平 (0 .0 36± 0 .0 0 6 ,P <0 .0 0 1)。灌流液中MCP 1蛋白时间依赖性增加 ,但 5h后增长趋缓 ;而在不同切应力 (0 .30、0 .72、2 .40Pa)相同时间 (5h)下 ,0 .72Pa的作用最显著 ,MCP 1蛋白比静态对照增加了 2倍多 ,mRNA水平则升高达 4倍。结论 MCP 1的表达对切应力的变化反应强烈 ,持续稳定的切应力则下调MCP 1的表达 ,此研究结果表明血流紊乱可促进AS病变的发生发展。
Objective To investigate the effect of fluid shear stress on the expression of monocyte chemoattractant protein 1 (MCP 1) in human vascular endothelial cells and to explore the role of hemodynamics in the early stage of atherosclerosis (AS). Methods Different shear stress was applied to the vascular endothelial cells by parallel plate flow chamber. The levels of MCP 1 protein and mRNA were detected by ELISA and RT-PCR respectively. The results were cut at 0 .72Pa shear stress for different time, MCP1 mRNA 0.5 h after static (0 16 0 ± 0 0 37) 4 times (0.84 ± 0. 033), 5h slightly (0. 0 36 ± 0. 0 0 6, P <0 0 0 1), which was significantly lower than that of the control group at 0 .70 7 ± 0. MCP 1 protein in perfusate increased in a time-dependent manner, but increased slowly after 5 h. However, the effect of 0.72 Pa was the most significant at the same time (5 h) with different shear stress (0.30, 0.72, 2.40 Pa) , MCP 1 protein increased more than 2 times than the static control, mRNA levels increased up to 4 times. Conclusions The expression of MCP 1 is strongly responsive to the change of shear stress. The sustained and stable shear stress decreases the expression of MCP 1. This result shows that the disturbance of blood flow can promote the development of AS.