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巨核细胞(Meg)的生物学研究比较困难,这是因为其量少,机械脆性大,且易聚集,故分离提取困难.迄今的分离方法如密度梯度离心法,逆向离心淘析法,所得纯度低(10-30%),回收率也低.新近的荧光激活细胞分提法,虽然快速且纯度高(90%),回收率20-40%,但不易普及.免疫磁珠法则可克服这些缺点,其敏感性高,快速且简便,尤其适用于量少的细胞和复杂细胞成分的分提.其方法是将骨髓细胞(BMC)置于两层不同密度(1.020和1.050g/ml)的Percoll液上,巨核细胞即居于大于1.020g/ml而小于1.050g/ml的组分中,经400g离心20分钟,弃上层液,收集中层(<1.050g/ml)细胞,用MK液洗涤2次,调整细胞浓度至1-5×
The biological study of meg megakaryocytes (Meg) is difficult due to its small amount, large mechanical fragility and easy aggregation, so it is difficult to separate and extract megakaryocytes (Meg), such as density gradient centrifugation, reversed-phase elutriation, and purity Low (10-30%), low recovery rate.New fluorescence-activated cell fractionation method, although fast and high purity (90%), 20-40% recovery rate is not easy to popularize. Immunomagnetic beads rules can overcome these It is sensitive, rapid and simple and is especially suitable for fractionation of cells and complex cell components in a small amount by placing bone marrow cells (BMCs) on two layers with different densities (1.020 and 1.050 g / ml) Percoll fluid, megakaryocytes that live in fractions greater than 1.020 g / ml but less than 1.050 g / ml are centrifuged at 400 g for 20 minutes. The supernatant is discarded and the middle (<1.050 g / ml) cells are harvested and washed with MK Times, adjust the cell concentration to 1-5 ×